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Product Info Summary
| SKU: | P02536 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Mouse, Rat |
| Host: | Sheep |
| Application: | ICC, WB |
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Product info
Product Name
Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody
SKU/Catalog Number
P02536
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody (Catalog # P02536). Tested in WB, ICC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02536)
Host
Sheep
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 of rat dynamin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P02536 is reactive to DNM1 in Mouse, Rat
Applications
P02536 is guaranteed for ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
97.408kDa
Background of Dynamin
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser-774 and Ser-778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser-774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
ICC: 1:1000
Validation Images & Assay Conditions
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Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at Ser774 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).
Protein Target Info & Infographic
Gene/Protein Information For DNM1 (Source: Uniprot.org, NCBI)
Gene Name
DNM1
Full Name
Dynamin-1
Weight
97.408kDa
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
DNM; DNM1; dynamin 1; Dynamin; dynamin-1; EC 3.6.5.5 DNM1 DEE31, DNM, EIEE31 dynamin 1 dynamin-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNM1, check out the DNM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody (P02536)
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No publications found for P02536
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody
Question
I was wanting to use your anti-Phospho-Dynamin (Ser774) antibody for WB for rat platelet on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat platelet identification?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
It shows on the product datasheet, P02536 anti-Phospho-Dynamin (Ser774) antibody has been validated for IF, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-31
Question
My question regarding product P02536, anti-Phospho-Dynamin (Ser774) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P02536 anti-Phospho-Dynamin (Ser774) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-12
Question
Would anti-Phospho-Dynamin (Ser774) antibody P02536 work for WB with platelet?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
According to the expression profile of platelet, DNM1 is highly expressed in platelet. So, it is likely that anti-Phospho-Dynamin (Ser774) antibody P02536 will work for WB with platelet.
Boster Scientific Support
Answered: 2019-11-04
Question
We have observed staining in rat brain pns. What should we do? Is anti-Phospho-Dynamin (Ser774) antibody supposed to stain brain pns positively?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
Based on literature brain pns does express DNM1. Based on Uniprot.org, DNM1 is expressed in frontal cortex, brain pns, cervix carcinoma, platelet, among other tissues. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-10-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Phospho-Dynamin (Ser774) antibody P02536. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
Does P02536 anti-Phospho-Dynamin (Ser774) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can see on the product datasheet, P02536 anti-Phospho-Dynamin (Ser774) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-12
Question
Do you have a BSA free version of anti-Phospho-Dynamin (Ser774) antibody P02536 available?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Dynamin (Ser774) antibody P02536 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-14
Question
See below the WB image, lot number and protocol we used for platelet using anti-Phospho-Dynamin (Ser774) antibody P02536. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-22
Question
I see that the anti-Phospho-Dynamin (Ser774) antibody P02536 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-15
Question
We are currently using anti-Phospho-Dynamin (Ser774) antibody P02536 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on feline tissues as well?
J. Anderson
Verified customer
Asked: 2018-10-10
Answer
The anti-Phospho-Dynamin (Ser774) antibody (P02536) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-10
Question
I am interested in to test anti-Phospho-Dynamin (Ser774) antibody P02536 on rat platelet for research purposes, then I may be interested in using anti-Phospho-Dynamin (Ser774) antibody P02536 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
The products we sell, including anti-Phospho-Dynamin (Ser774) antibody P02536, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-31
Question
Is this P02536 anti-Phospho-Dynamin (Ser774) antibody reactive to the isotypes of DNM1?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
The immunogen of P02536 anti-Phospho-Dynamin (Ser774) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser774 of rat dynamin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-01
Question
We ordered your anti-Phospho-Dynamin (Ser774) antibody for IF on frontal cortex a few months ago. I am using rat, and I plan to use the antibody for WB next. you antibody examining frontal cortex as well as platelet in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
I viewed the website and datasheets of our anti-Phospho-Dynamin (Ser774) antibody and it appears that P02536 has been validated on rat in both IF and WB. Thus P02536 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-20
Question
Is a blocking peptide available for product anti-Phospho-Dynamin (Ser774) antibody (P02536)?
R. Jackson
Verified customer
Asked: 2015-04-06
Answer
We do provide the blocking peptide for product anti-Phospho-Dynamin (Ser774) antibody (P02536). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-04-06
Question
My colleagues were happy with the WB result of your anti-Phospho-Dynamin (Ser774) antibody. However we have seen positive staining in platelet cytoplasm. using this antibody. Is that expected? Could you tell me where is DNM1 supposed to be expressed?
L. Evans
Verified customer
Asked: 2014-04-30
Answer
From literature, platelet does express DNM1. Generally DNM1 expresses in cytoplasm. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2014-04-30
