Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody

Dynamin antibody

Boster Bio Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody catalog # A02536-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A02536-Dyl550
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

Customers Who Bought This Also Bought

Product Name

Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody

View all Dynamin Antibodies

SKU/Catalog Number

A02536-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody catalog # A02536-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02536-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Dynamin 1 recombinant protein (Position: W616-D667).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A02536-Dyl550 is reactive to DNM1 in Human

Applications

A02536-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Background of Dynamin

Dynamin-1 is a protein that in humans is encoded by the DNM1 gene. This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For DNM1 (Source: Uniprot.org, NCBI)

Uniprot ID

Q05193

Gene ID

1759

Gene Name

DNM1

Full Name

Dynamin-1

Weight

97.408kDa

Superfamily

TRAFAC class dynamin-like GTPase superfamily

Alternative Names

DNM; DNM1; dynamin 1; Dynamin; dynamin-1; EC 3.6.5.5 DNM1 DEE31, DNM, EIEE31 dynamin 1 dynamin-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DNM1, check out the DNM1 Infographic

DNM1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DNM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

No publications found for A02536-Dyl550

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

14 Customer Q&As for Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody

Question

Will anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550 work for Flow Cytometry with frontal cortex?

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

According to the expression profile of frontal cortex, DNM1 is highly expressed in frontal cortex. So, it is likely that anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550 will work for Flow Cytometry with frontal cortex.

Boster Scientific Support

Answered: 2020-05-08

Question

My question regarding product A02536-Dyl550, anti-Human Dynamin 1 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02536-Dyl550 anti-Human Dynamin 1 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-20

Question

I see that the anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

N. Williams

Verified customer

Asked: 2020-03-23

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-23

Question

We are currently using anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

The anti-Human Dynamin 1 DyLight® 550 conjugated antibody (A02536-Dyl550) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-19

Question

Would A02536-Dyl550 anti-Human Dynamin 1 DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

As indicated on the product datasheet, A02536-Dyl550 anti-Human Dynamin 1 DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-19

Question

Is this A02536-Dyl550 anti-Human Dynamin 1 DyLight® 550 conjugated antibody reactive to the isotypes of DNM1?

A. Moore

Verified customer

Asked: 2019-09-12

Answer

The immunogen of A02536-Dyl550 anti-Human Dynamin 1 DyLight® 550 conjugated antibody is E. coli-derived human Dynamin 1 recombinant protein (Position: W616-D667). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-12

Question

Here is the WB image, lot number and protocol we used for frontal cortex using anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-21

Question

Is a blocking peptide available for product anti-Human Dynamin 1 DyLight® 550 conjugated antibody (A02536-Dyl550)?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

We do provide the blocking peptide for product anti-Human Dynamin 1 DyLight® 550 conjugated antibody (A02536-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-21

Question

My boss were well pleased with the WB result of your anti-Human Dynamin 1 DyLight® 550 conjugated antibody. However we have seen positive staining in frontal cortex cytoplasm. using this antibody. Is that expected? Could you tell me where is DNM1 supposed to be expressed?

A. Li

Verified customer

Asked: 2018-02-08

Answer

According to literature, frontal cortex does express DNM1. Generally DNM1 expresses in cytoplasm. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2018-02-08

Question

I am interested in to test anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550 on human frontal cortex for research purposes, then I may be interested in using anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-28

Answer

The products we sell, including anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-28

Question

Is there a BSA free version of anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550 available?

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-05-24

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for frontal cortex using anti-Human Dynamin 1 DyLight® 550 conjugated antibody A02536-Dyl550. Let me know if you need anything else.

Z. Johnson

Verified customer

Asked: 2016-02-15

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-02-15

Question

We have observed staining in human frontal cortex. Do you have any suggestions? Is anti-Human Dynamin 1 DyLight® 550 conjugated antibody supposed to stain frontal cortex positively?

N. Jha

Verified customer

Asked: 2014-08-19

Answer

According to literature frontal cortex does express DNM1. According to Uniprot.org, DNM1 is expressed in frontal cortex, brain pns, cervix carcinoma, platelet, among other tissues. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2014-08-19

Question

I was wanting to use your anti-Human Dynamin 1 DyLight® 550 conjugated antibody for Flow Cytometry for human frontal cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human frontal cortex identification?

M. Parker

Verified customer

Asked: 2014-03-03

Answer

As indicated on the product datasheet, A02536-Dyl550 anti-Human Dynamin 1 DyLight® 550 conjugated antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human frontal cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-03-03

Size

Total: $515

SKU:A02536-Dyl550

Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

Product Categories

Primary Antibodies

Primary and Secondary Antibodies

Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!

ELISA Kits

ELISA Kits

More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

Image

Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

Boster Kit

Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

In stock
Order Product
A02536-Dyl550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.