Anti-Dystroglycan DAG1 Antibody

Product Name

Anti-Dystroglycan DAG1 Antibody

View all DAG1 Antibodies

SKU/Catalog Number

A01369

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Dystroglycan DAG1 Antibody catalog # A01369. Tested in WB,ICC/IF,IP applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Dystroglycan DAG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01369)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from part region of human protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01369 is reactive to DAG1 in Human, Mouse, Rat

Applications

A01369 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

97.441kDa

Background of DAG1

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

Kumagai H., Mol. Cell. Biol. 19:5083-5095(1999).
Jiang W., EMBO J. 18:5703-5713(1999).
Ota T., Nat. Genet. 36:40-45(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
ICC/IF: 1:20-1:100
IP: 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-Dystroglycan DAG1 Antibody A01369

Western blot analysis of extracts of various cells

Gene/Protein Information For DAG1 (Source: Uniprot.org, NCBI)

Alternative Names

A3a; AGRNR; DAG156DAG; dystroglycan 1 (dystrophin-associated glycoprotein 1); dystroglycan; Dystrophin-associated glycoprotein 1 DAG1 156DAG, A3a, AGRNR, DAG, LGMDR16, MDDGA9, MDDGC7, MDDGC9 dystroglycan 1 dystroglycan|dystroglycan 1 (dystrophin-associated glycoprotein 1)

For more info on DAG1, check out the DAG1 Infographic

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In this infographic, you will see the following information for DAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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