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Product Info Summary
| SKU: | A04191 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-EIF2C3/Argonaute 3 AGO3 Antibody
SKU/Catalog Number
A04191
Size
100ul
Form
Liquid
Description
AGO3 (EIF2C3) is an Argonaute-family protein required for RNA-mediated gene silencing; it binds short RNAs (e.g., miRNAs) and contributes to post-transcriptional repression as part of RISC-like complexes (mechanistic specifics vary by small-RNA context). Assay context: Human/Mouse/Rat-reactive antibody validated for WB, IHC, ICC/IF, and flow cytometry; in cell-based quantification workflows, Argonaute abundance is also compatible with in-cell western blot style readouts (putative). Often compared directly with AGO2 as the canonical RISC core component.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF2C3/Argonaute 3 AGO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04191)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
recombinant protein
Reactive Species
A04191 is reactive to AGO3 in Human, Mouse, Rat
Calculated molecular weight
97.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04191 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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ICC staining EIF2C3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining EIF2C3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Specific Publications For Anti-EIF2C3/Argonaute 3 AGO3 Antibody (A04191)
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