Product Info Summary
SKU: | A30682 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-EIF3F/Eif3 Epsilon Antibody
SKU/Catalog Number
A30682
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EIF3F/Eif3 Epsilon Antibody (Catalog# A30682). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF3F/Eif3 Epsilon Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30682)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human EIF3F. Immunogen sequence location: 81-130
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30682 is reactive to EIF3F in Human, Mouse, Rat
Applications
A30682 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
37.564kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HeLa cells, using EIF3F Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EIF3F Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For EIF3F (Source: Uniprot.org, NCBI)
Gene Name
EIF3F
Full Name
Eukaryotic translation initiation factor 3 subunit F
Weight
37.564kDa
Superfamily
eIF-3 subunit F family
Alternative Names
eIF3 p47; eIF-3-epsilon; eIF3-epsilon; eIF3f; eIF3-p47; EIF3S5eukaryotic translation initiation factor 3 subunit F; Eukaryotic translation initiation factor 3 subunit 5; eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD); eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; eukaryotic translation initiation factor 3, subunit F EIF3F EIF3S5, MRT67, eIF3-p47 eukaryotic translation initiation factor 3 subunit F eukaryotic translation initiation factor 3 subunit F|deubiquitinating enzyme eIF3f|eIF-3-epsilon|eIF3-epsilon|eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD)|eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF3F, check out the EIF3F Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF3F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EIF3F/Eif3 Epsilon Antibody (A30682)
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No publications found for A30682
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-EIF3F/Eif3 Epsilon Antibody
Question
We are currently using anti-EIF3F/Eif3 Epsilon antibody A30682 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
The anti-EIF3F/Eif3 Epsilon antibody (A30682) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-03