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Product Info Summary
| SKU: | A31838 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ERRF SRARP Antibody
SKU/Catalog Number
A31838
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ERRF SRARP Antibody (Catalog # A31838). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ERRF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ERRF SRARP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31838)
Host
Rabbit
Contents
ERRF Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ERRF antibody was raised against a 15 amino acid peptide near the carboxy terminus of human ERRF . The immunogen is located within the last 50 amino acids of ERRF.
Reactive Species
A31838 is reactive to C1ORF64 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
17.7 kDa
Background of C1ORF64
The estrogen receptor-related factor (ERRF) is a recently identified protein whose expression is upregulated in multiple cell lines and primary breast cancer tumors. Increased ERRF expression was significantly associated with ER or progesterone receptor (PR) positivity even in HER2-negative tumors. This higher ERRF expression correlated with better disease-free survival and overall survival. In two ER-positive breast cancer cell lines, knockdown of ERRF suppressed cell growth in vitro and tumorigenesis in xenograft models, suggesting that ERRF plays a role in estrogen-ER-mediated breast cancer cell growth and may thus be a potential therapeutic target.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A31838 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ERRF Antibody can be used for detection of ERRF by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ERRF in (A) HeLa and (B) A-20 cell lysate with ERRF antibody at 1 μg/mL.
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Immunocytochemistry of ERRF in HeLa cells with ERRF antibody at 2.5 μg/ml.
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Immunofluorescence of ERRF in HeLa cells with ERRF antibody at 20 μg/ml.
Specific Publications For Anti-ERRF SRARP Antibody (A31838)
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