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Product Info Summary
| SKU: | M00102-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-IL-6 Monoclonal Antibody
SKU/Catalog Number
M00102-1
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-IL-6 Monoclonal Antibody (Catalog # M00102-1). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store IL 6 antibody at -20°C . For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IL-6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00102-1)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 33A12.G9
Isotype
IgG1 kappa
Immunogen
This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.
Cross-reactivity
Weakly cross-reacts with human PDGF receptor β.
Reactive Species
M00102-1 is reactive to IL6 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
23.7 kDa
Background of IL6
IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody is ideal for investigators involved in Cancer, Neuroscience and Immunology research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00102-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000
Flow Cytometry: 0.5 mg/mL
WB: 1:1000
Anti-IL-6 antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Validation Images & Assay Conditions
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Anti-Human IL-6 Antibody - Flow Cytometry. Human PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours. Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly. 1mL of permeabilization buffer was added. 0.5mg of Anti-Human IL-6 Antibody was added (0.125 ug/mL control antibody) and incubated in the dark for 30 minutes. 1:100 of strep/PE was added and incubated for 30 minutes. LPS-stimulated samples were compared to unstimulated cells stained with strep/PE.
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Flow Cytometry of Human anti-IL-6 antibody 209-301-310. Cells: human PBMC. Stimulation: Figure A: unstimulated; Figure B: 1 µg/mL LPS in a protein transport inhibitor for 5 hours. Staining: (surface) x-axis: anti-CD14, (intracellular) y-axis: anti-IL-6.
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Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20°C, Anti-Human IL-6 (MOUSE) Antibody was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.
Specific Publications For Anti-IL-6 Monoclonal Antibody (M00102-1)
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Customer Q&As
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15 Customer Q&As for Anti-IL-6 Monoclonal Antibody
Question
I see that the anti-IL-6 Monoclonal antibody M00102-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-17
Question
I was wanting to use your anti-IL-6 Monoclonal antibody for Flow Cytometry for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
D. Collins
Verified customer
Asked: 2019-11-11
Answer
It shows on the product datasheet, M00102-1 anti-IL-6 Monoclonal antibody has been tested for Flow Cytometry, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-11
Question
I am interested in to test anti-IL-6 Monoclonal antibody M00102-1 on human lung for research purposes, then I may be interested in using anti-IL-6 Monoclonal antibody M00102-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The products we sell, including anti-IL-6 Monoclonal antibody M00102-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-10
Question
We have observed staining in human left coronary artery. What should we do? Is anti-IL-6 Monoclonal antibody supposed to stain left coronary artery positively?
A. Carter
Verified customer
Asked: 2019-09-26
Answer
Based on literature left coronary artery does express IL6. Based on Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-26
Question
Is this M00102-1 anti-IL-6 Monoclonal antibody reactive to the isotypes of IL6?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
The immunogen of M00102-1 anti-IL-6 Monoclonal antibody is This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-20
Question
My colleagues were happy with the WB result of your anti-IL-6 Monoclonal antibody. However we have seen positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
Based on literature, lung does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-02-20
Question
See below the WB image, lot number and protocol we used for lung using anti-IL-6 Monoclonal antibody M00102-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-18
Question
Can you help my question with product M00102-1, anti-IL-6 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00102-1 anti-IL-6 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-12
Question
Would M00102-1 anti-IL-6 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
You can see on the product datasheet, M00102-1 anti-IL-6 Monoclonal antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-28
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-IL-6 Monoclonal antibody M00102-1. Let me know if you need anything else.
E. Anderson
Verified customer
Asked: 2017-10-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-16
Question
Is a blocking peptide available for product anti-IL-6 Monoclonal antibody (M00102-1)?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
We do provide the blocking peptide for product anti-IL-6 Monoclonal antibody (M00102-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-08-11
Question
Does anti-IL-6 Monoclonal antibody M00102-1 work for Flow Cytometry with lung?
V. Rodriguez
Verified customer
Asked: 2016-08-01
Answer
According to the expression profile of lung, IL6 is highly expressed in lung. So, it is likely that anti-IL-6 Monoclonal antibody M00102-1 will work for Flow Cytometry with lung.
Boster Scientific Support
Answered: 2016-08-01
Question
Is there a BSA free version of anti-IL-6 Monoclonal antibody M00102-1 available?
A. Yang
Verified customer
Asked: 2016-02-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IL-6 Monoclonal antibody M00102-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-24
Question
We ordered your anti-IL-6 Monoclonal antibody for Flow Cytometry on lung in a previous experiment. I am using human, and We want to use the antibody for WB next. I am looking for examining lung as well as fibroblast in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
G. Li
Verified customer
Asked: 2014-03-27
Answer
I took a look at the website and datasheets of our anti-IL-6 Monoclonal antibody and it appears that M00102-1 has been tested on human in both Flow Cytometry and WB. Thus M00102-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-03-27
Question
We are currently using anti-IL-6 Monoclonal antibody M00102-1 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
J. Zhang
Verified customer
Asked: 2013-03-27
Answer
The anti-IL-6 Monoclonal antibody (M00102-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-27
