Anti-Factor B/CFB Antibody Picoband™

Product Name

Anti-Factor B/CFB Antibody Picoband™

View all Complement Factor B Antibodies

SKU/Catalog Number

PB9934

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Factor B/CFB Antibody Picoband™ catalog # PB9934. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Factor B/CFB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9934)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Factor B recombinant protein (Position: E518-L764). Human Factor B shares 84.2% amino acid (aa) sequence identity with mouse Factor B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9934 is reactive to CFB in Human

Applications

PB9934 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

86 kDa

Calculated molecular weight

85.533kDa

Background of Complement Factor B

Complement factor B is a protein that in humans is encoded by the CFB gene. This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Factor B using anti-Factor B antibody (PB9934).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor B antigen affinity purified polyclonal antibody (Catalog # PB9934) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor B at approximately 86 kDa. The expected band size for Factor B is at 86 kDa.

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Figure 2. IHC analysis of Factor B using anti-Factor B antibody (PB9934).
Factor B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Factor B Antibody (PB9934) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For CFB (Source: Uniprot.org, NCBI)

Alternative Names

BF AHUS4; BF; B-factor, properdin; BFD; C3 proaccelerator; C3 proactivator; C3/C5 convertase; CFAB; CFB; Complement Factor B; EC 3.4.21; EC 3.4.21.47; FB; FBI12; GBG; GBGCFAB; Glycine-rich beta glycoprotein; glycine-rich beta-glycoprotein; H2-Bf; PBF2; Properdin factor B CFB AHUS4, ARMD14, BF, BFD, CFABD, FB, FBI12, GBG, H2-Bf, PBF2, CFB complement factor B complement factor B|B-factor, properdin|C3 proaccelerator|C3 proactivator|C3/C5 convertase|glycine-rich beta-glycoprotein|properdin factor B

For more info on CFB, check out the CFB Infographic

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In this infographic, you will see the following information for CFB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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