Anti-FAM129B/NIBAN2 Antibody Picoband™

Product Name

Anti-FAM129B/NIBAN2 Antibody Picoband™

View all NIBAN2 Antibodies

SKU/Catalog Number

A09391-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FAM129B/NIBAN2 Antibody Picoband™ catalog # A09391-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-FAM129B/NIBAN2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09391-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FAM129B/NIBAN2 recombinant protein (Position: K23-K561).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09391-2 is reactive to NIBAN2 in Human, Mouse, Rat

Applications

A09391-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

84.138kDa

Background of NIBAN2

FAM129B/Niban-like protein 1 (family with sequence similarity 129, member B) belongs to a poorly characterized family of Niban proteins that also includes FAM129A/Niban and FAM129C/Niban-like protein 2. FAM129A/Niban is implicated in the ER stress response and is upregulated at the protein level in thyroid carcinoma. FAM129C/Niban-like protein 2 is preferentially expressed in B-cells and is one of five biomarkers upregulated in whole blood from patients with colorectal carcinoma. FAM129B is broadly expressed and has been shown to be a downstream target of B-Raf in melanoma cells. Though FAM129B does not appear to regulate cell growth and division, phosphorylation of FAM129B by B-Raf is essential for the invasive potential of melanoma and non-melanoma cell lines. Deletion of FAM129B in melanoma cells significantly impairs B-Raf/MEK/Erk-dependent invasion into the extracellular matrix.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FAM129B/NIBAN2 using anti-FAM129B/NIBAN2 antibody (A04887-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM129B/NIBAN2 antigen affinity purified polyclonal antibody (Catalog # A04887-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM129B/NIBAN2 at approximately 95 kDa. The expected band size for FAM129B/NIBAN2 is at 95 kDa.

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Figure 2. IHC analysis of FAM129B/NIBAN2 using anti-FAM129B/NIBAN2 antibody (A09391-2).
FAM129B/NIBAN2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM129B/NIBAN2 Antibody (A09391-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of FAM129B/NIBAN2 using anti-FAM129B/NIBAN2 antibody (A09391-2).
FAM129B/NIBAN2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM129B/NIBAN2 Antibody (A09391-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 4. Flow Cytometry analysis of Hela cells using anti-FAM129B/NIBAN2 antibody (A09391-2).
Overlay histogram showing Hela cells stained with A09391-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM129B/NIBAN2 Antibody (A09391-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For NIBAN2 (Source: Uniprot.org, NCBI)

Alternative Names

Protein Niban 2 NIBAN2 C9orf88, FAM129B, MEG-3, MINERVA, OC58, bA356B19.6 niban apoptosis regulator 2 protein Niban 2|family with sequence similarity 129 member B|melanoma invasion by ERK|niban-like protein 1

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In this infographic, you will see the following information for NIBAN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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