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Product Info Summary
| SKU: | A01932 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-FIS1 Antibody
SKU/Catalog Number
A01932
Size
100ul
Form
Liquid
Description
Boster Bio Anti-FIS1 Antibody catalog # A01932. Tested in WB,ICC/IF,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FIS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01932)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human TTC11/FIS1(NP_057152.2).
Reactive Species
A01932 is reactive to FIS1 in Human, Mouse, Rat
Calculated molecular weight
16.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01932 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of 293T cells, using TTC11/FIS1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
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Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 3ug TTC11/FIS1 antibody . Western blot was performed from the immunoprecipitate using TTC11/FIS1 antibody at a dilution of 1:1000.
Specific Publications For Anti-FIS1 Antibody (A01932)
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