Product Info Summary
SKU: | M02199 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-G3BP Rabbit Monoclonal Antibody
SKU/Catalog Number
M02199
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-G3BP Rabbit Monoclonal Antibody catalog # M02199. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-G3BP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02199)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22G81
Isotype
IgG
Immunogen
A synthesized peptide derived from human G3BP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02199 is reactive to G3BP1 in Human, Mouse, Rat
Applications
M02199 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.164kDa
Background of G3BP1
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of G3BP expression in Hela cell lysate.
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Immunocytochemistry analysis of N2A cells, using G3BP antibody.
Click image to see more details
All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For G3BP1 (Source: Uniprot.org, NCBI)
Gene Name
G3BP1
Full Name
Ras GTPase-activating protein-binding protein 1
Weight
52.164kDa
Alternative Names
ATP-dependent DNA helicase VIII; EC 3.6.1; EC 3.6.4.12; EC 3.6.4.13; G3BP-1; G3BPRas-GTPase-activating protein SH3-domain-binding protein; GAP binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; ras GTPase-activating protein-binding protein 1; RasGAP-associated endoribonuclease G3BP G3BP1 G3BP, HDH-VIII G3BP stress granule assembly factor 1 ras GTPase-activating protein-binding protein 1|ATP-dependent DNA helicase VIII|DNA helicase VIII|G3BP-1|GAP SH3 domain-binding protein 1|GAP binding protein|GTPase activating protein (SH3 domain) binding protein 1|Ras-GTPase-activating protein SH3-domain-binding protein|RasGAP-associated endoribonuclease G3BP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on G3BP1, check out the G3BP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for G3BP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-G3BP Rabbit Monoclonal Antibody (M02199)
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No publications found for M02199
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Customer Reviews
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