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Product Info Summary
| SKU: | A04752 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GABRB1(Ab-434) Antibody
View all GABA-A R beta 1 Antibodies
SKU/Catalog Number
A04752
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GABRB1(Ab-434) Antibody (Catalog # A04752). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GABRB1(Ab-434) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04752)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.432~436 (R-A-S-Q-L) derived from Human GABRB1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04752 is reactive to GABRB1 in Human, Mouse, Rat
Applications
A04752 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.235kDa
Background of GABA-A R beta 1
The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 1 subunit. It is mapped to chromosome 4p12 in a cluster comprised of genes encoding a 4, a 2 and gamma 1 subunits of the GABA A receptor. Alteration of this gene is implicated in the pathogenetics of schizophrenia.
Collins AL, et al. (2006) Neurogenetics ;7(3):167-174.
Ma DQ, et al. (2005) Am J Hum Genet ;77(3):377-388.
Edenberg HJ, et al. (2004) Am J Hum Genet ;74(4):705-714.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 54kd
Western blotting: 1:500~1:1000
IHC 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts from PC12 cells using GABRB1(Ab-434) antibody A04752 and the same antibody preincubated with blocking peptide.
Protein Target Info & Infographic
Gene/Protein Information For GABRB1 (Source: Uniprot.org, NCBI)
Gene Name
GABRB1
Full Name
Gamma-aminobutyric acid receptor subunit beta-1
Weight
54.235kDa
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
GABA A R beta 1; GABA(A) receptor subunit beta-1; GABAAR beta 1; GABAARb1; GABRB1; gamma-aminobutyric acid (GABA) A receptor, beta 1; gamma-aminobutyric acid receptor subunit beta-1 GABRB1 DEE45, EIEE45 gamma-aminobutyric acid type A receptor subunit beta1 gamma-aminobutyric acid receptor subunit beta-1|gamma-aminobutyric acid (GABA) A receptor, beta 1|gamma-aminobutyric acid type A receptor beta1 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GABRB1, check out the GABRB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GABRB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GABRB1(Ab-434) Antibody (A04752)
Hello CJ!
No publications found for A04752
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-GABRB1(Ab-434) Antibody
Question
My question regards to test anti-GABRB1(Ab-434) antibody A04752 on human brain for research purposes, then I may be interested in using anti-GABRB1(Ab-434) antibody A04752 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
The products we sell, including anti-GABRB1(Ab-434) antibody A04752, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-08
Question
Would anti-GABRB1(Ab-434) antibody A04752 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
According to the expression profile of brain, GABRB1 is highly expressed in brain. So, it is likely that anti-GABRB1(Ab-434) antibody A04752 will work for WB with brain.
Boster Scientific Support
Answered: 2020-03-20
Question
Is this A04752 anti-GABRB1(Ab-434) antibody reactive to the isotypes of GABRB1?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The immunogen of A04752 anti-GABRB1(Ab-434) antibody is Peptide sequence around aa.432~436 (R-A-S-Q-L) derived from Human GABRB1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-03
Question
I was wanting to use your anti-GABRB1(Ab-434) antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
It shows on the product datasheet, A04752 anti-GABRB1(Ab-434) antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-30
Question
We are currently using anti-GABRB1(Ab-434) antibody A04752 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
C. Mangal
Verified customer
Asked: 2019-12-17
Answer
The anti-GABRB1(Ab-434) antibody (A04752) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-17
Question
I have attached the WB image, lot number and protocol we used for brain using anti-GABRB1(Ab-434) antibody A04752. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-27
Question
Will anti-GABRB1(Ab-434) antibody A04752 work on primate WB with brodmann (1909) area 23?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Our lab technicians have not validated anti-GABRB1(Ab-434) antibody A04752 on primate. You can run a BLAST between primate and the immunogen sequence of anti-GABRB1(Ab-434) antibody A04752 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brodmann (1909) area 23 in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-22
Question
Is a blocking peptide available for product anti-GABRB1(Ab-434) antibody (A04752)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We do provide the blocking peptide for product anti-GABRB1(Ab-434) antibody (A04752). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-27
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-GABRB1(Ab-434) antibody A04752. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-08
Question
I see that the anti-GABRB1(Ab-434) antibody A04752 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-20
Question
Can you help my question with product A04752, anti-GABRB1(Ab-434) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04752 anti-GABRB1(Ab-434) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-01
Question
Do you have a BSA free version of anti-GABRB1(Ab-434) antibody A04752 available?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GABRB1(Ab-434) antibody A04752 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-12
Question
Does A04752 anti-GABRB1(Ab-434) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
It shows on the product datasheet, A04752 anti-GABRB1(Ab-434) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-30
