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Product Info Summary
| SKU: | A06627 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Galactokinase GALK1 Antibody
SKU/Catalog Number
A06627
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Galactokinase GALK1 Antibody (Catalog # A06627). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galactokinase GALK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06627)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human GALK1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06627 is reactive to GALK1 in Human, Mouse, Rat
Applications
A06627 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
42.272kDa
Background of GALK1
Major enzyme for galactose metabolism.
Stambolian D., Nat. Genet. 10:307-312(1995).
Bergsma D.J., Genome Res. 6:980-985(1996).
Ai Y., Biochem. Biophys. Res. Commun. 212:687-691(1995).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HUVEC cells and HeLa cells, using GALK1 antibody A06627.
Protein Target Info & Infographic
Gene/Protein Information For GALK1 (Source: Uniprot.org, NCBI)
Gene Name
GALK1
Full Name
Galactokinase
Weight
42.272kDa
Superfamily
GHMP kinase family
Alternative Names
EC 2.7.1.6; galactokinase 1; galactokinase; Galactose kinase; GALK; GK1 GALK1 GALK, GK1, HEL-S-19 galactokinase 1 galactokinase|epididymis secretory protein Li 19|galactose kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GALK1, check out the GALK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GALK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Galactokinase GALK1 Antibody (A06627)
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No publications found for A06627
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-Galactokinase GALK1 Antibody
Question
Does anti-GALK1 antibody A06627 work for WB with lung?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
According to the expression profile of lung, GALK1 is highly expressed in lung. So, it is likely that anti-GALK1 antibody A06627 will work for WB with lung.
Boster Scientific Support
Answered: 2019-10-23
Question
We are currently using anti-GALK1 antibody A06627 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
The anti-GALK1 antibody (A06627) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-23
Question
Can you help my question with product A06627, anti-GALK1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06627 anti-GALK1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-GALK1 antibody A06627. Let me know if you need anything else.
T. Zhao
Verified customer
Asked: 2015-06-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-16
