Anti-GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker) Monoclonal Antibody

Product Name

Anti-GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker) Monoclonal Antibody

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SKU/Catalog Number

M04543

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker) Monoclonal Antibody (Catalog # M04543). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04543)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: PIP/1571

Isotype

IgG2a, kappa

Immunogen

Recombinant fragment (around aa 41-146) of human GCDFP-15 protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M04543 is reactive to PIP in Human

Applications

M04543 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

16.572kDa

Background of PIP

This monoclonal antibody recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen-controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.Â

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Western Blot analysis (A) Human Pancreas (B) HepG2 cell lysate using Anti-GCDFP-15 Mouse Monoclonal Antibody (PIP/1571).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Anti-GCDFP-15 Mouse Monoclonal Antibody (PIP/1571).

Gene/Protein Information For PIP (Source: Uniprot.org, NCBI)

Alternative Names

GCDFP15prolactin-inducible protein; GCDFP-15SABP; GPIP4gp17; Gross cystic disease fluid protein 15; prolactin-induced proteinSecretory actin-binding protein PIP GCDFP-15, GCDFP15, GPIP4 prolactin induced protein prolactin-inducible protein|SABP|gross cystic disease fluid protein 15|secretory actin-binding protein

For more info on PIP, check out the PIP Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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