SKU H30939
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number DG-7
Host Rabbit
Applications WB

Overview

Product Name Anti-GFP Rabbit Monoclonal Antibody, HRP Conjugated
SKU/Catalog Number H30939
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-GFP Rabbit Monoclonal Antibody, HRP Conjugated tested for WB in Human, Mouse, Rat
Cite This Product Anti-GFP Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H30939)
Host Rabbit
Immunogen A synthesized peptide
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

WB 1:3000-1:10000

Images And Assay Conditions

/H/3/H30939 GFP primary antibodies WB testing 1.jpg

Western blot analysis of GFP fusion protein expression in (1) 293T cell lysate; (2) 293T cell transfected GFP fusion protein with GFP Antibody(HRP conjugated) (H30939).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFP monoclonal antibody (Catalog # H30939) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFP

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q963I9
Gene Name GFP
Alternative Names Green fluorescent protein ;GFP ;
Molecular Weight 25993 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Order Product (H30939)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

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