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Product Info Summary
| SKU: | A01710-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Ghrelin/GHRL Antibody Picoband™
SKU/Catalog Number
A01710-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ghrelin/GHRL Antibody Picoband™ catalog # A01710-2. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Ghrelin/GHRL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01710-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Ghrelin/GHRL, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01710-2 is reactive to GHRL in Human
Applications
A01710-2 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
20 kDa
Calculated molecular weight
12.911kDa
Background of GHRL
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Ghrelin/GHRL using anti-Ghrelin/GHRL antibody (A01710-2).
Ghrelin/GHRL was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ghrelin/GHRL Antibody (A01710-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GHRL (Source: Uniprot.org, NCBI)
Gene Name
GHRL
Full Name
Appetite-regulating hormone
Weight
12.911kDa
Superfamily
motilin family
Alternative Names
appetite-regulating hormone; Ghrelin; ghrelin/obestatin preprohormone; ghrelin/obestatin prepropeptide; GHRL; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related Peptide; MTLRPgrowth hormone secretagogue receptor ligand; obestatin; Protein M46 GHRL MTLRP ghrelin and obestatin prepropeptide appetite-regulating hormone|In2c-preproghrelin|ghrelin, growth hormone secretagogue receptor ligand|ghrelin/obestatin preprohormone|ghrelin/obestatin prepropeptide|growth hormone-releasing peptide|motilin-related peptide|prepro-appetite regulatory hormone|preproghrelin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GHRL, check out the GHRL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GHRL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ghrelin/GHRL Antibody Picoband™ (A01710-2)
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No publications found for A01710-2
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Customer Reviews
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