Product Info Summary
SKU: | A07972-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-GLI-1 ACSS1 Antibody
SKU/Catalog Number
A07972-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GLI-1 ACSS1 Antibody catalog # A07972-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLI-1 ACSS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07972-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized Acetyl peptide derived from Human ACSS1 at AA range: K642.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07972-1 is reactive to ACSS1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
62989 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07972-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of 1) mouse heart, 2) HeLa cells using primary antibody diluted at 1: 500 (4℃ overnight) . Secondary antibody: Goat Anti-rabbit IgG IRDye 800 (diluted at 1: 5000, 25℃, 1 hour).
Protein Target Info & Infographic
Gene/Protein Information For ACSS1 (Source: Uniprot.org, NCBI)
Gene Name
ACSS1
Full Name
Acetyl-coenzyme A synthetase 2-like, mitochondrial
Weight
62989 MW
Superfamily
ATP-dependent AMP-binding enzyme family
Alternative Names
cDNA FLJ57539, highly similar to Zinc finger protein GLI1 ; Acss1|1110032O15Rik, AI788978, Ac, Acas2, Acas2l, AceC, AceCS2|acyl-CoA synthetase short-chain family member 1|acetyl-coenzyme A synthetase 2-like, mitochondrial|acetate--CoA ligase 2|acetyl-CoA synthetase 2|propionate--CoA ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACSS1, check out the ACSS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACSS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLI-1 ACSS1 Antibody (A07972-1)
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No publications found for A07972-1
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Customer Q&As
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6 Customer Q&As for Anti-GLI-1 ACSS1 Antibody
Question
I was wanting to use your anti-GLI-1 antibody for WB for human thalamus tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human thalamus tongue identification?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
You can see on the product datasheet, A07972-1 anti-GLI-1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human thalamus tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-06
Question
Will anti-GLI-1 antibody A07972-1 work on monkey WB with thalamus tongue?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Our lab technicians have not validated anti-GLI-1 antibody A07972-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-GLI-1 antibody A07972-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey thalamus tongue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thalamus tongue using anti-GLI-1 antibody A07972-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-28
Question
Here is the WB image, lot number and protocol we used for thalamus tongue using anti-GLI-1 antibody A07972-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-10
Question
Is there a BSA free version of anti-GLI-1 antibody A07972-1 available?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GLI-1 antibody A07972-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-04
Question
We are currently using anti-GLI-1 antibody A07972-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
The anti-GLI-1 antibody (A07972-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-28