Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody

Glypican 3 antibody

Boster Bio Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody (Catalog # M01922). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: M01922
Size: 100ug/vial
Reactive Species: Human, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC

Product Name

Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody

View all Glypican 3 Antibodies

SKU/Catalog Number

M01922

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody (Catalog # M01922). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01922)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: rGPC3/863

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human GPC3 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01922 is reactive to GPC3 in Human, Rat

Applications

M01922 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

65.563kDa

Background of Glypican 3

Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For GPC3 (Source: Uniprot.org, NCBI)

Gene Name

GPC3

Full Name

Glypican-3

Weight

65.563kDa

Superfamily

glypican family

Alternative Names

DGSX; Glypican 3; glypican proteoglycan 3; glypican-3; GPC3; GTR2-2; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI5; OCI-5; secreted glypican-3; SGB; SGBS; SGBS1SDYS GPC3 DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1 glypican 3 glypican-3|glypican proteoglycan 3|heparan sulphate proteoglycan|intestinal protein OCI-5|secreted glypican-3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GPC3, check out the GPC3 Infographic

GPC3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody

Question

Is this M01922 anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody reactive to the isotypes of GPC3?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

The immunogen of M01922 anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody is Recombinant full-length human GPC3 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-16

Question

See below the WB image, lot number and protocol we used for embryo using anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody M01922. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-29

Question

Would M01922 anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-06-07

Answer

You can see on the product datasheet, M01922 anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-06-07

Question

I have a question about product M01922, anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Lewis

Verified customer

Asked: 2018-03-26

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01922 anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-03-26

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryo using anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody M01922. Let me know if you need anything else.

R. Mangal

Verified customer

Asked: 2014-11-28

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-11-28

Question

Does anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody M01922 work on primate IHC with embryo?

S. Jones

Verified customer

Asked: 2013-02-04

Answer

Our lab technicians have not validated anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody M01922 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal antibody M01922 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate embryo in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-02-04

Order DetailsPrice
M01922-100ug

100 μg

$468

Free Secondary Antibody

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M01922
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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