Anti-GNG4 Antibody Picoband™ (monoclonal, 7B6)

Product Name

Anti-GNG4 Antibody Picoband™ (monoclonal, 7B6)

View all G gamma4 Antibodies

SKU/Catalog Number

M13925

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GNG4 Antibody Picoband™ (monoclonal, 7B6) catalog # M13925. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GNG4 Antibody Picoband™ (monoclonal, 7B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13925)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

7B6

Isotype

Mouse IgG1

Immunogen

E.coli-derived human GNG4 recombinant protein (Position: M1-D52).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M13925 is reactive to GNG4 in Mouse, Rat

Applications

M13925 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

12 kDa

Calculated molecular weight

8.389kDa

Background of G gamma4

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 is a protein that in humans is encoded by the GNG4 gene. This gene encodes the gamma subunit of the heterotrimeric G-proteins that are comprised of alpha, beta and gamma subunits. Upon activation by G protein-coupled receptors, the beta-gamma heterodimer dissociates from the alpha subunit to activate downstream signaling events. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GNG4 using anti-GNG4 antibody (M13925).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNG4 antigen affinity purified monoclonal antibody (Catalog # M13925) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GNG4 at approximately 12 kDa. The expected band size for GNG4 is at 12 kDa.

Gene/Protein Information For GNG4 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp547K1018; FLJ23803; FLJ34187; GNGT4; guanine nucleotide binding protein (G protein), gamma 4; guanine nucleotide binding protein 4; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 GNG4 G protein subunit gamma 4 guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4|guanine nucleotide binding protein (G protein), gamma 4|guanine nucleotide binding protein 4

For more info on GNG4, check out the GNG4 Infographic

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In this infographic, you will see the following information for GNG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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