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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-GPR161 Antibody Picoband™
Boster Bio Anti-GPR161 Antibody Picoband™ catalog # A10567-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GPR161 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10567-1)
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
A synthetic peptide corresponding to a sequence of human GPR161(LHVKAEVHKSLDSYAASLAKAIEAEAKINL).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A10567-1 is reactive to GPR161 in Human, Mouse, Rat
A10567-1 is guaranteed for WB Boster Guarantee
Background of GPR161
G-protein coupled receptor 161 is a protein that in humans is encoded by the GPR161 gene. The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GPR161 using anti-GPR161 antibody (A10567-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: huamn SiHa whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR161 antigen affinity purified polyclonal antibody (Catalog # A10567-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPR161 at approximately 60 kDa. The expected band size for GPR161 is at 60 kDa.
Gene/Protein Information For GPR161 (Source: Uniprot.Org, NCBI)
G-protein coupled receptor 161
G-protein coupled receptor 1 family
FLJ33952; G protein-coupled receptor 161; Gm208; GPR161; G-protein coupled receptor 161; G-protein coupled receptor RE2; RE2 GPR161 RE2 G protein-coupled receptor 161 G-protein coupled receptor 161|G-protein coupled receptor RE2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on GPR161, check out the GPR161 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GPR161: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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