Product Info Summary
SKU: | A13015 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-G-protein coupled receptor 52 GPR52 Antibody
SKU/Catalog Number
A13015
Size
100μl
Form
Liquid
Description
Boster Bio Anti-G-protein coupled receptor 52 GPR52 Antibody catalog # A13015. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-G-protein coupled receptor 52 GPR52 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13015)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2H4
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human GPR52. at AA rangle: 270-350
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13015 is reactive to GPR52 in Human
Applications
A13015 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.354kDa
Background of GPR52
COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of 293T 3T3 lysis using GPR52 antibody.
Click image to see more details
Western Blot (WB) analysis of specific cells using GPR52 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For GPR52 (Source: Uniprot.org, NCBI)
Gene Name
GPR52
Full Name
G-protein coupled receptor 52
Weight
41.354kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
G protein-coupled receptor 52; GPR52; MGC111751; probable G-protein coupled receptor 52 GPR52 G protein-coupled receptor 52 G-protein coupled receptor 52|probable G-protein coupled receptor 52
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPR52, check out the GPR52 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPR52: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-G-protein coupled receptor 52 GPR52 Antibody (A13015)
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No publications found for A13015
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Recommended Resources
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-G-protein coupled receptor 52 GPR52 Antibody
Question
I was wanting to use your anti-GPR52 antibody for WB for human caudate nucleus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human caudate nucleus identification?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
It shows on the product datasheet, A13015 anti-GPR52 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human caudate nucleus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-20
Question
We are currently using anti-GPR52 antibody A13015 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
J. Lewis
Verified customer
Asked: 2018-01-04
Answer
The anti-GPR52 antibody (A13015) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-04
Question
My question regarding product A13015, anti-GPR52 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jones
Verified customer
Asked: 2016-09-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13015 anti-GPR52 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-09-14
Question
We want to test anti-GPR52 antibody A13015 on human caudate nucleus for research purposes, then I may be interested in using anti-GPR52 antibody A13015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Zhang
Verified customer
Asked: 2015-09-14
Answer
The products we sell, including anti-GPR52 antibody A13015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-09-14