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Product Info Summary
| SKU: | A05402-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Heat shock 70 kDa protein 6 HSPA6 Antibody
SKU/Catalog Number
A05402-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Heat shock 70 kDa protein 6 HSPA6 Antibody catalog # A05402-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heat shock 70 kDa protein 6 HSPA6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05402-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human HSPA6(NP_002146.2).
Reactive Species
A05402-1 is reactive to HSPA6 in Human, Mouse, Rat
Observed Molecular Weight
111 kDa
Calculated molecular weight
71.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05402-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using HSPA6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human liver using HSPA6 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Specific Publications For Anti-Heat shock 70 kDa protein 6 HSPA6 Antibody (A05402-1)
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