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Product Info Summary
| SKU: | A03805-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Hif3 alpha Antibody
SKU/Catalog Number
A03805-1
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Hif3 alpha Antibody (Catalog # A03805-1). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Hif3 alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03805-1)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor).
Reactive Species
A03805-1 is reactive to HIF3A in Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
73.0 kDa
Background of HIF3A
One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF binds to the hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and apoptosis. Hif3 may participate in disorders with the cornea, lung, and heart. Anti-Hif3 alpha Antibody is useful for researchers interested in transcription factors, DNA binding, and Epilepsy research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03805-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:14,000 - 1:80,000
WB: 1:1,000 - 1:8,000
This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 alpha protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 alpha due to sequence homology.
Validation Images & Assay Conditions
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Western blot using Boster's Affinity Purified Anti-Hif3A antibody shows detection of a band ~72 kDa corresponding to mouse Hif3A [arrowhead]. Approximately 10 µg of a CoCl2 treated 3T3 cell lysate [lane 1] and control 3T3 cell lysate [lane 2] were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 µM CoCl2 for 6 h at 37° C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4° C with the primary antibody diluted to 1:1,600. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Specific Publications For Anti-Hif3 alpha Antibody (A03805-1)
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