Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M30949 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Histone H1 (Nuclear Marker) Monoclonal Antibody
SKU/Catalog Number
M30949
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Histone H1 (Nuclear Marker) Monoclonal Antibody (Catalog # M30949). Tested in WB, Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Histone H1 (Nuclear Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30949)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: r1415-1
Isotype
IgG2a, kappa
Immunogen
Recombinant full-length human Histone H1 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M30949 is reactive to in Human, Mouse, Rat
Applications
M30949 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
29159 MW
Background of
Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
Click image to see more details
Formalin-fixed, paraffin-embedded Rat Pancreas stained with Anti-Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
Click image to see more details
Immunofluorescent staining of PFA-fixed HeLa cells using Anti-Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1) followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.
Click image to see more details
Western Blot analysis of human heart tissue lysate using Anti-Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
Click image to see more details
Flow Cytometric analysis of HeLa cells. Anti-Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Specific Publications For Anti-Histone H1 (Nuclear Marker) Monoclonal Antibody (M30949)
Hello CJ!
M30949 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Role of endoplasmic reticulum stress in apoptosis of differentiated mouse podocytes induced by high glucose
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Histone H1 (Nuclear Marker) Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Histone H1 (Nuclear Marker) Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Histone H1 (Nuclear Marker) Monoclonal Antibody
Question
Will anti-Histone H1 (Nuclear Marker) Monoclonal antibody M30949 work on feline IHC with colon placenta?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Our lab technicians have not tested anti-Histone H1 (Nuclear Marker) Monoclonal antibody M30949 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Histone H1 (Nuclear Marker) Monoclonal antibody M30949 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline colon placenta in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-10
Question
Can you help my question with product M30949, anti-Histone H1 (Nuclear Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M30949 anti-Histone H1 (Nuclear Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-28
Question
See attached the WB image, lot number and protocol we used for brain using anti-Histone H1 (Nuclear Marker) Monoclonal antibody M30949. Please let me know if you require anything else.
M. Dhar
Verified customer
Asked: 2015-01-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-27
