Click image to see more details
Product Info Summary
| SKU: | M16777-4 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody, Clone#RM225
SKU/Catalog Number
M16777-4
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody, Clone#RM225 (Catalog # M16777-4). Tested in WB, ICC. ELISA, Luminex applications. This antibody reacts with Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody, Clone#RM225 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M16777-4)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM225
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human Histone H2A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the Histone H2A protein, independent of post-translational modifications. No cross-reactivity with other histone proteins.
Reactive Species
M16777-4 is reactive to H2AC4 in Vertebrates
Applications
M16777-4 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
14.135kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5 ug/mL – 2 ug/mL
ICC: 1 μg/mL - 2 μg/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.2 ug/mL – 1 ug/mL.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM225 at 0.5 ug/mL
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Figure 2. ICC result
Immunocytochemistry of HeLa cells, using Histone H2A Rabbit mAb RM225 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Protein Target Info & Infographic
Gene/Protein Information For H2AC4 (Source: Uniprot.org, NCBI)
Gene Name
H2AC4
Full Name
Histone H2A type 1-B/E
Weight
14.135kDa
Superfamily
histone H2A family
Alternative Names
Histone H2A type 1-B/E H2AC4 H2A/m, H2AC8, H2AFM, HIST1H2AB H2A clustered histone 4 histone H2A type 1-B/E|H2A histone family, member M|histone 1, H2ab|histone H2A/m|histone cluster 1 H2A family member b|histone cluster 1, H2ab
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H2AC4, check out the H2AC4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H2AC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody, Clone#RM225 (M16777-4)
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No publications found for M16777-4
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody, Clone#RM225
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for stomach using anti-Histone H2A Rabbit Monoclonal antibody, Clone#RM225 M16777-4. Let me know if you need anything else.
L. Jones
Verified customer
Asked: 2016-11-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-11-28
Question
Can you help my question with product M16777-4, anti-Histone H2A Rabbit Monoclonal antibody, Clone#RM225. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Carter
Verified customer
Asked: 2016-06-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M16777-4 anti-Histone H2A Rabbit Monoclonal antibody, Clone#RM225, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-29
Question
Please see the WB image, lot number and protocol we used for stomach using anti-Histone H2A Rabbit Monoclonal antibody, Clone#RM225 M16777-4. Please let me know if you require anything else.
A. Baker
Verified customer
Asked: 2016-04-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-04-06
