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Product Info Summary
| SKU: | M08989 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Histone H2B Rabbit Monoclonal Antibody, Clone#RM230
SKU/Catalog Number
M08989
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Histone H2B Rabbit Monoclonal Antibody, Clone#RM230 (Catalog # M08989). Tested in WB, ICC. ELISA, Luminex applications. This antibody reacts with Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2B Rabbit Monoclonal Antibody, Clone#RM230 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08989)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM230
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human Histone H2B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the Histone H2B protein, independent of post-translational modifications. No cross-reactivity with other histone proteins.
Reactive Species
M08989 is reactive to H2BC11 in Vertebrates
Applications
M08989 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
13.904kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.1 ug/mL – 1 ug/mL
ICC: 0.5 μg/mL - 1 μg/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.2 ug/mL – 1 ug/mL.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM230 at 0.2 ug/mL
Click image to see more details
Figure 2. ICC result
Immunocytochemical staining of HeLa cells, using Histone H2B Rabbit mAb RM230 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Protein Target Info & Infographic
Gene/Protein Information For H2BC11 (Source: Uniprot.org, NCBI)
Gene Name
H2BC11
Full Name
Histone H2B type 1-J
Weight
13.904kDa
Superfamily
histone H2B family
Alternative Names
Histone H2B type 1-J H2BC11 H2B/r, H2BFR, H2BJ, HIST1H2BJ H2B clustered histone 11 histone H2B type 1-J|H2B histone family, member R|histone 1, H2bj|histone H2B.1|histone H2B.r|histone cluster 1 H2B family member j|histone cluster 1, H2bj
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H2BC11, check out the H2BC11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H2BC11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H2B Rabbit Monoclonal Antibody, Clone#RM230 (M08989)
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No publications found for M08989
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Histone H2B Rabbit Monoclonal Antibody, Clone#RM230
Question
We are currently using anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 M08989 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
S. Dhar
Verified customer
Asked: 2019-12-17
Answer
The anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 (M08989) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-17
Question
Is a blocking peptide available for product anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 (M08989)?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
We do provide the blocking peptide for product anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 (M08989). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-31
Question
Will anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 M08989 work on pig WB with hippocampus?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
Our lab technicians have not tested anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 M08989 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 M08989 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig hippocampus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-17
Question
I have a question about product M08989, anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08989 anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-06
Question
Our lab want to know about to test anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 M08989 on human stomach for research purposes, then I may be interested in using anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 M08989 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Mitchell
Verified customer
Asked: 2018-07-13
Answer
The products we sell, including anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 M08989, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-13
Question
I was wanting to use your anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 for ICC for human stomach on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human stomach identification?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
As indicated on the product datasheet, M08989 anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 has been tested for ELISA, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human stomach in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for stomach using anti-Histone H2B Rabbit Monoclonal antibody, Clone#RM230 M08989. Let me know if you need anything else.
M. Johnson
Verified customer
Asked: 2015-11-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-11-27
