Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody

Boster Bio Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (Catalog # M30948). Tested in ELISA, Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M30948
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: ELISA, Flow Cytometry, IF, IHC, WB

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Product Name

Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody

View all Antibodies

SKU/Catalog Number

M30948

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (Catalog # M30948). Tested in ELISA, Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30948)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: CR3/43

Isotype

IgG1, kappa

Immunogen

Cells from human tonsil

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M30948 is reactive to in Human

Applications

M30948 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

29159 MW

Background of

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (For coating use Ab at 2-4ug/ml, order Ab without BSA)
Flow Cytometry (1-2ug/ million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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5 Customer Q&As for Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody

Question

Do you have a BSA free version of anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948 available?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-05

Question

Will anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948 work for IHC with blood?

Verified Customer

Verified customer

Asked: 2019-05-20

Answer

According to the expression profile of blood, HLA-DPB1 is highly expressed in blood. So, it is likely that anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948 will work for IHC with blood.

Boster Scientific Support

Answered: 2019-05-20

Question

See attached the WB image, lot number and protocol we used for blood using anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-15

Question

Can you help my question with product M30948, anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Baker

Verified customer

Asked: 2015-04-01

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M30948 anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-04-01

Question

Is this M30948 anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody reactive to the isotypes of HLA-DPB1?

D. Jha

Verified customer

Asked: 2013-07-30

Answer

The immunogen of M30948 anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody is Cells from human tonsil. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-07-30

Order DetailsPrice
M30948-100ug

100 μg

$468

Free Secondary Antibody

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M30948
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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