Product Info Summary
SKU: | M30948 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody
SKU/Catalog Number
M30948
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (Catalog # M30948). Tested in ELISA, Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30948)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: CR3/43
Isotype
IgG1, kappa
Immunogen
Cells from human tonsil
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M30948 is reactive to in Human
Applications
M30948 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
29159 MW
Background of
Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (For coating use Ab at 2-4ug/ml, order Ab without BSA)
Flow Cytometry (1-2ug/ million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-HLA-Pan Mouse Monoclonal Antibody (CR3/43).
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-HLA-Pan Mouse Monoclonal Antibody (CR3/43).
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Flow Cytometric analysis of Human Raji cells using Anti-HLA-Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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Immunofluorescent staining of Raji cells. Anti-HLA-Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
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Western Blot analysis of Ramos cell lysate using Anti-HLA-Pan Mouse Monoclonal Antibody (CR3/43).
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Western Blot analysis of Human Spleen cell lysate using Anti-HLA-Pan Mouse Monoclonal Antibody (CR3/43).
Specific Publications For Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (M30948)
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No publications found for M30948
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-HLA-DP/-DQ/-DR (MHC II) HLA-DPB1 Monoclonal Antibody
Question
Do you have a BSA free version of anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948 available?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-05
Question
Will anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948 work for IHC with blood?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
According to the expression profile of blood, HLA-DPB1 is highly expressed in blood. So, it is likely that anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948 will work for IHC with blood.
Boster Scientific Support
Answered: 2019-05-20
Question
See attached the WB image, lot number and protocol we used for blood using anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody M30948. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-15
Question
Can you help my question with product M30948, anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Baker
Verified customer
Asked: 2015-04-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M30948 anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-04-01
Question
Is this M30948 anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody reactive to the isotypes of HLA-DPB1?
D. Jha
Verified customer
Asked: 2013-07-30
Answer
The immunogen of M30948 anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal antibody is Cells from human tonsil. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-07-30