Anti-HLA-DP/-DR (MHC II) HLA-DPB1 Monoclonal Antibody

Product Name

Anti-HLA-DP/-DR (MHC II) HLA-DPB1 Monoclonal Antibody

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SKU/Catalog Number

M00487

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-HLA-DP/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (Catalog # M00487). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-HLA-DP/-DR (MHC II) HLA-DPB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00487)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: Bra-14

Isotype

IgG3, kappa

Immunogen

Human REH cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00487 is reactive to in Human

Applications

M00487 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

29159 MW

Background of

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with 1ml water (15 minutes at room temperature).

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-HLA-DP/DR Mouse Monoclonal Antibody (Bra-14).

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