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Product Info Summary
| SKU: | A01322 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Homeobox protein Nkx-2.1 NKX2-1 Antibody
View all TTF-1 / NKX2-1 Antibodies
SKU/Catalog Number
A01322
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Homeobox protein Nkx-2.1 NKX2-1 Antibody (Catalog # A01322). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NKX2-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Homeobox protein Nkx-2.1 NKX2-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01322)
Host
Rabbit
Contents
NKX2-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
NKX2-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1. The immunogen is located within the first 50 amino acids of NKX2-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three.
Reactive Species
A01322 is reactive to NKX2-1 in Human, Mouse, Rat
Applications
A01322 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
38.596kDa
Background of TTF-1 / NKX2-1
NKX2-1 (NK2 homeobox 1) has been identified as a thyroid-specific transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype and morphogenesis. It contains one homeobox DNA-binding domain. NKX2-1 also may play a role in lung development and surfactant homeostasis. Mutations and deletions in this gene are associated with benign hereditary chorea, congenital hypothyroidism, neonatal respiratory distress, and may be associated with thyroid cancer. NKX2-1 has been suggested to be a candidate suppressor of malignant progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NKX2-1 antibody can be used for detection of NKX2-1 by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NKX2-1 expression in human lung tissue lysate with NKX2-1 antibody at (A) 0.25 and (B) 0.5 μg/ml.
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Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 μg/mL.
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Immunofluorescence of NKX2-1 in human lung tissue with NKX2-1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NKX2-1 (Source: Uniprot.org, NCBI)
Gene Name
NKX2-1
Full Name
Homeobox protein Nkx-2.1
Weight
38.596kDa
Superfamily
NK-2 homeobox family
Alternative Names
BCH; benign chorea; BHC; Homeobox protein NK-2 homolog A; homeobox protein Nkx-2.1; NK2 homeobox 1; NK-2 homolog A; NKX2.1; NKX2-1; NKX2A; NKX2ANKX2.1; T/EBP; Thyroid nuclear factor 1; Thyroid transcription factor 1BHC; TITF1; TITF1TEBP; TTF1; TTF-1; TTF1BCH; TTF-1NK-2 NKX2-1 BCH, BHC, NK-2, NKX2.1, NKX2A, NMTC1, T/EBP, TEBP, TITF1, TTF-1, TTF1 NK2 homeobox 1 homeobox protein Nkx-2.1|NK-2 homolog A|homeobox protein NK-2 homolog A|thyroid nuclear factor 1|thyroid transcription factor 1|thyroid-specific enhancer-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NKX2-1, check out the NKX2-1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NKX2-1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein Nkx-2.1 NKX2-1 Antibody (A01322)
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No publications found for A01322
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