Anti-HR23A RAD23A Antibody

Product Name

Anti-HR23A RAD23A Antibody

View all Rad23 Antibodies

SKU/Catalog Number

A03243

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HR23A RAD23A Antibody (Catalog # A03243). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-HR23A RAD23A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03243)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 115-140 of human HR23A protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03243 is reactive to RAD23A in Human

Applications

A03243 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

39.609kDa

Background of Rad23

HR23A (also known as UV excision repair protein RAD23 homolog A) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:16,000
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of HR23A expression in HELA nuclear extract (lane 1), HELA whole cell lysates (lane 2), A431 whole cell lysates (lane 3) JURKAT whole cell lysates (lane 4) and 293 whole cell lysates (lane 5). HR23A at 60KD was detected using goat anti-HR23A Antigen Affinity purified polyclonal antibody (Catalog # A03243) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1003).

Gene/Protein Information For RAD23A (Source: Uniprot.org, NCBI)

Alternative Names

hHR23; Rad23 RAD23A HHR23A, HR23A RAD23 homolog A, nucleotide excision repair protein UV excision repair protein RAD23 homolog A|RAD23, yeast homolog, A

For more info on RAD23A, check out the RAD23A Infographic

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In this infographic, you will see the following information for RAD23A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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