Anti-Hu CD111 Purified NECTIN1 Monoclonal Antibody

Product Name

Anti-Hu CD111 Purified NECTIN1 Monoclonal Antibody

View all Nectin-1/PVRL1 Antibodies

SKU/Catalog Number

M04988

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD111 Purified NECTIN1 Monoclonal Antibody (Catalog# M04988). Tested in Flow Cytometry, IP, WB, IHC, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD111 Purified NECTIN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04988)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

R1.302

Isotype

Mouse IgG1 kappa

Immunogen

NIH/3T3 cells transfected with human CD111. The mouse monoclonal antibody R1.302 recognizes an extracellular epitope on CD111 (also known as Nectin 1), a 75 kDa type I transmembrane glycoprotein broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M04988 is reactive to NECTIN1 in Human

Applications

M04988 is guaranteed for Flow Cytometry, IP, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

57.158kDa

Background of Nectin-1/PVRL1

CD111, also known as nectin-1, is a calcium-independent cell-cell adhesion transmembrane glycoprotein involved in organization of adherens junctions and tight junctions in epithelial and endothelial cells. It also serves as a target molecule for entry of herpes simplex virus (HSV-1, HSV-2) and pseudorabies virus (PRV) into epithelial and neuronal cells. CD111 is connected with actin cytoskeleton through afadin. Mutations in the gene for CD111 cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of NECTIN1 using Anti-Hu CD111 Purified NECTIN1 Monoclonal Antibody (M04988).

Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD111 (R1.302) purified

Gene/Protein Information For NECTIN1 (Source: Uniprot.org, NCBI)

Alternative Names

CD111 antigen; CD111; CLPED1ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgRPVRR; HVEC; HVECpoliovirus receptor-like 1; MGC142031; Nectin1; Nectin-1; OFC7nectin 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; PVRR1nectin-1; SK-12 NECTIN1 CD111, CLPED1, ED4, HIgR, HV1S, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK-12, nectin-1 nectin cell adhesion molecule 1 nectin-1|ectodermal dysplasia 4 (Margarita Island type)|herpes simplex virus type 1 sensitivity|herpes virus entry mediator C|herpesvirus Ig-like receptor|nectin 1|poliovirus receptor-like 1|poliovirus receptor-related 1 (herpesvirus entry mediator C)|poliovirus receptor-related protein 1

For more info on NECTIN1, check out the NECTIN1 Infographic

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In this infographic, you will see the following information for NECTIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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