Anti-Hu CD15 Purified FUT4 Monoclonal Antibody

Product Name

Anti-Hu CD15 Purified FUT4 Monoclonal Antibody

View all SSEA-1 Antibodies

SKU/Catalog Number

M04535-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD15 Purified FUT4 Monoclonal Antibody (Catalog# M04535-1). Tested in Flow Cytometry, IHC-P, IHC-F, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD15 Purified FUT4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04535-1)

Host

Mouse

Contents

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MMA

Isotype

Mouse IgM kappa

Immunogen

U937 histiocytic lymphoma cells. The mouse monoclonal antibody MMA reacts with an extracellular epitope of CD15, a cell membrane 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. This antibody is a superior reagent for identifying of Hodgkin´s lymphoma.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M04535-1 is reactive to FUT4 in Human

Applications

M04535-1 is guaranteed for Flow Cytometry, IHC-P, IHC-F, ICC Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

59.084kDa

Background of SSEA-1

CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of FUT4 using Anti-Hu CD15 Purified FUT4 Monoclonal Antibody (M04535-1).

Flow cytometry analysis (surface staining) of CD15 in human peripheral blood with anti-CD15 (MMA) purified

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Figure 2. Immunohistochemistry validation of FUT4 using Anti-Hu CD15 Purified FUT4 Monoclonal Antibody (M04535-1).

Immunohistochemistry staining of human uterus (paraffin-embedded sections) with anti-CD15 (MMA)

Gene/Protein Information For FUT4 (Source: Uniprot.org, NCBI)

Alternative Names

alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1 FUT4 CD15, ELFT, FCT3A, FUC-TIV, FUTIV, LeX, SSEA-1 fucosyltransferase 4 alpha-(1,3)-fucosyltransferase 4|ELAM ligand fucosyltransferase|ELAM-1 ligand fucosyltransferase|Lewis X|alpha (1,3) fucosyltransferase, myeloid-specific|fucT-IV|fucosyltransferase IV|galactoside 3-L-fucosyltransferase|stage-specific embryonic antigen 1

For more info on FUT4, check out the FUT4 Infographic

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In this infographic, you will see the following information for FUT4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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