Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody

Product Name

Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody

View all KIR2DL5/CD158f Antibodies

SKU/Catalog Number

M12423

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody (Catalog# M12423). Tested in Flow Cytometry, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12423)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

UP-R1

Isotype

Mouse IgG1 kappa

Immunogen

Human CD158f-Ig fusion protein. The mouse monoclonal antibody UP-R1 recognizes an extracellular epitope on CD158f (KIR2DL5), a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M12423 is reactive to KIR2DL5A in Human

Applications

M12423 is guaranteed for Flow Cytometry, ICC Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

40.678kDa

Background of KIR2DL5/CD158f

CD158f, also known as KIR2DL5, is a polymorphic 60 kDa transmembrane glycoprotein with two Ig-like extracellular domains by which it recognize HLA class I molecules. Its long intracellular domain contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that upon extracellular ligand-mediated phosphorylation serve as docking sites for inhibitory phosphatases, which results in blocking natural cytotoxicity as well as antibody-dependent cytotoxicity of the particular NK cell, and its adhesion toward target cells. Together with other killer inhibitory receptors CD158f is important for immunological tolerance to discriminate between normal and abnormal cells. Besides NK cells it is expressed on a small population of cytotoxic T cells. Expression of CD158f alleles is highly variable in the population.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of KIR2DL5A using Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody (M12423).

Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD158f (UP-R1) purified / GAM-APC.

Gene/Protein Information For KIR2DL5A (Source: Uniprot.org, NCBI)

Alternative Names

CD158f; CD158F1; killer cell immunoglobulin-like receptor 2DL5A; killer cell immunoglobulin-like receptor KIR2DL5A; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A; killer-cell Ig-like receptor; KIR2DL5; KIR2DL5.1; KIR2DL5.3; KIR2DL5A; KIR2DL5B KIR2DL5A CD158F, KIR2DL5, KIR2DL5.1, KIR2DL5.3 killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 5A killer cell immunoglobulin-like receptor 2DL5A|killer cell immunoglobulin-like receptor KIR2DL5A|killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5|killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A

For more info on KIR2DL5A, check out the KIR2DL5A Infographic

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In this infographic, you will see the following information for KIR2DL5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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