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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Rat IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat IL-1 Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il1a in cell culture supernatants and serum. Sensitivity: 0.5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0390)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: S115-S270
4.69 pg/ml - 300 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0390 is reactive to Il1a in Rat samples
Validated Sample Types
cell culture supernatants and serum.
EK0390 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-1 alpha/IL-1F1
Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18kDa.
|EK0390-CAP||Anti-Rat Il1a Pre-coated 96-well strip microplate||1|
|EK0390-ST||Rat Il1a Standard||2 vials, 10 ng/tube|
|EK0390-DA||Rat Il1a Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat IL-1 alpha PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-1 alpha/IL-1F1, Dilution ratio of 1:1.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IL-1 alpha/IL-1F1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Il1a (Source: Uniprot.Org, NCBI)
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha Il1a|IL-1 alpha, IL-1F1|interleukin 1 alpha|interleukin-1 alpha*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il1a, check out the Il1a Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il1a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0390 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li Y, Wang N, Ma Z, Wang Y, Yuan Y, Zhong Z, Hong Y, Zhao M. Lipoxin A4 protects against paraquat‑induced acute lung injury by inhibiting the TLR4/MyD88‑mediated activation of the NF‑κB and PI3K/AKT pathways. Int J Mol Med. 2021 May;47(5):86. doi: 10.3892/ijmm. 2021.4919. Epub 2021 Mar 24. PMID: 33760150.
EK0390 usage in article: APP:ELISA, SAMPLE:BALF AND RAW264.7 CELL, DILUTION:NA
He C,Su C,Zhang W,Zhou Q,Shen X,Yang J,Shi N.Modulatory Potential of LncRNA Zfas1 for Inflammation and Neuronal Apoptosis in Temporal Lobe Epilepsy.Yonsei Med J.2021 Mar;62(3):215-223.doi:10.3349/ymj.2021.62.3.215.PMID:33635011.
EK0390 usage in article: APP:ELISA, SAMPLE:NEURON, DILUTION:NA
Urinary brain-derived neurotrophic factor: a potential biomarker for objective diagnosis of overactive bladder
Cytokine Attenuation and Free Radical Scavenging Activity of a New Flavanone7,4?-Dihydroxy-3?,3?-Dimethyl -(5,6-Pyrano-2?-One)- 8- (3?,3?-Dimethyl Allyl)- Isolated from Mallotus philippensis: Possible Mechanism for Its Anti-Inflammatory Activity
Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells
Suppression of complete Freund's adjuvant-induced adjuvant arthritis by cobratoxin
Rizvi W, Fayazuddin M, Singh O, Syed SN, Moin S, Akhtar K, Kumar A. Avicenna J Phytomed. 2017 Jan-Feb;7(1):37-45. Anti-inflammatory effect of Fumaria parviflora leaves based on TNF-α, IL-1, IL-6 and antioxidant potential
Zhang L, Xu C, Hu W, Wu P, Qin C, Zhang J. Int J Mol Med. 2018 Mar;41(3):1293-1304. doi: 10.3892/ijmm.2017.3327. Epub 2017 Dec 18. Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway
Rizvi W, Fayazuddin M, Shariq S, Singh O, Moin S, Akhtar K, Kumar A. Anc Sci Life. 2014 Jul-Sep;34(1):44-9. Doi: 10.4103/0257-7941.150780. Anti-Inflammatory Activity Of Roots Of Cichorium Intybus Due To Its Inhibitory Effect On Various Cytokines A...
Zhou Qb, Jin Yl, Jia Q, Zhang Y, Li Ly, Liu P, Liu Yt. Inflammation. 2014 Feb;37(1):107-15. Doi: 10.1007/S10753-013-9717-9. Baicalin Attenuates Brain Edema In A Rat Model Of Intracerebral Hemorrhage.
Li J, Huang M, Shen X. J Diabetes Complications. 2014 Sep-Oct;28(5):662-6. Doi: 10.1016/J.Jdiacomp.2014.06.008. Epub 2014 Jun 19. The Association Of Oxidative Stress And Pro-Inflammatory Cytokines In Diabetic Patients With Hyperglycemic Crisis.
Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo...
Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli...
Duan F, Liao J, Huang Q, Nie Y, Wu K. Clin Dev Immunol. 2012;2012:192791. Doi: 10.1155/2012/192791. Epub 2012 Apr 9. Hsv-1 Mir-H6 Inhibits Hsv-1 Replication And Il-6 Expression In Human Corneal Epithelial Cells In Vitro.
Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.
Shi Q, Xie Wl, Zhang B, Chen Ln, Xu Y, Wang K, Ren K, Zhang Xm, Chen C, Zhang J, Dong Xp. Virol J. 2013 Jul 1;10:216. Doi: 10.1186/1743-422X-10-216. Brain Microglia Were Activated In Sporadic Cjd But Almost Unchanged In Fatal Familial Insomnia And...
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