Rat IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™

Rat IL-1 Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il1a in cell culture supernatants and serum. Sensitivity: 0.5pg/ml. Cited in 16 publication(s).

Product Info Summary

SKU: EK0390
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Rat IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™

See all IL-1 alpha/IL-1F1 products

SKU/Catalog Number

EK0390

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-1 Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il1a in cell culture supernatants and serum. Sensitivity: 0.5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0390)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S115-S270

Sensitivity

<0.5 pg/ml

Assay Range

4.69 pg/ml - 300 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0390 is reactive to Il1a in Rat samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0390 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0390-CAP Anti-Rat Il1a Pre-coated 96-well strip microplate 1
EK0390-ST Rat Il1a Standard 2 vials, 10 ng/tube
EK0390-DA Rat Il1a Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)04.699.418.837.575150300
O.D.0.0720.1170.1990.2490.6781.0901.4342.227

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-1 alpha/IL-1F1, Dilution ratio of 1:1.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)15401311443120
Standard deviation1.062.129.691.092.5810.8
CV (%)7.1%5.3%7.4%7.8%6%9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-1 alpha/IL-1F1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 115161517150.825.4%
Sample 240394645423.047.2%
Sample 31311231461361348.336.2%
*number of samples for each test n=16.

Gene/Protein Information For Il1a (Source: Uniprot.Org, NCBI)

Uniprot ID

P16598

Gene ID

24493

Gene Name

Il1a

Full Name

Interleukin-1 alpha

Weight

30.856kDa

Superfamily

IL-1 family

Alternative Names

BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha Il1a|IL-1 alpha, IL-1F1|interleukin 1 alpha|interleukin-1 alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il1a, check out the Il1a Infographic

Il1a infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il1a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0390 has been cited in 16 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li Y, Wang N, Ma Z, Wang Y, Yuan Y, Zhong Z, Hong Y, Zhao M. Lipoxin A4 protects against paraquat‑induced acute lung injury by inhibiting the TLR4/MyD88‑mediated activation of the NF‑κB and PI3K/AKT pathways. Int J Mol Med. 2021 May;47(5):86. doi: 10.3892/ijmm. 2021.4919. Epub 2021 Mar 24. PMID: 33760150.
Species: Mouse,Rat
EK0390 usage in article: APP:ELISA, SAMPLE:BALF AND RAW264.7 CELL, DILUTION:NA

He C,Su C,Zhang W,Zhou Q,Shen X,Yang J,Shi N.Modulatory Potential of LncRNA Zfas1 for Inflammation and Neuronal Apoptosis in Temporal Lobe Epilepsy.Yonsei Med J.2021 Mar;62(3):215-223.doi:10.3349/ymj.2021.62.3.215.PMID:33635011.
Species: Human,Rat
EK0390 usage in article: APP:ELISA, SAMPLE:NEURON, DILUTION:NA

Urinary brain-derived neurotrophic factor: a potential biomarker for objective diagnosis of overactive bladder

Cytokine Attenuation and Free Radical Scavenging Activity of a New Flavanone7,4?-Dihydroxy-3?,3?-Dimethyl -(5,6-Pyrano-2?-One)- 8- (3?,3?-Dimethyl Allyl)- Isolated from Mallotus philippensis: Possible Mechanism for Its Anti-Inflammatory Activity

Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells

Suppression of complete Freund's adjuvant-induced adjuvant arthritis by cobratoxin

Rizvi W, Fayazuddin M, Singh O, Syed SN, Moin S, Akhtar K, Kumar A. Avicenna J Phytomed. 2017 Jan-Feb;7(1):37-45. Anti-inflammatory effect of Fumaria parviflora leaves based on TNF-α, IL-1, IL-6 and antioxidant potential

Zhang L, Xu C, Hu W, Wu P, Qin C, Zhang J. Int J Mol Med. 2018 Mar;41(3):1293-1304. doi: 10.3892/ijmm.2017.3327. Epub 2017 Dec 18. Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway

Rizvi W, Fayazuddin M, Shariq S, Singh O, Moin S, Akhtar K, Kumar A. Anc Sci Life. 2014 Jul-Sep;34(1):44-9. Doi: 10.4103/0257-7941.150780. Anti-Inflammatory Activity Of Roots Of Cichorium Intybus Due To Its Inhibitory Effect On Various Cytokines A...

Zhou Qb, Jin Yl, Jia Q, Zhang Y, Li Ly, Liu P, Liu Yt. Inflammation. 2014 Feb;37(1):107-15. Doi: 10.1007/S10753-013-9717-9. Baicalin Attenuates Brain Edema In A Rat Model Of Intracerebral Hemorrhage.

Li J, Huang M, Shen X. J Diabetes Complications. 2014 Sep-Oct;28(5):662-6. Doi: 10.1016/J.Jdiacomp.2014.06.008. Epub 2014 Jun 19. The Association Of Oxidative Stress And Pro-Inflammatory Cytokines In Diabetic Patients With Hyperglycemic Crisis.

Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo...

Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli...

Duan F, Liao J, Huang Q, Nie Y, Wu K. Clin Dev Immunol. 2012;2012:192791. Doi: 10.1155/2012/192791. Epub 2012 Apr 9. Hsv-1 Mir-H6 Inhibits Hsv-1 Replication And Il-6 Expression In Human Corneal Epithelial Cells In Vitro.

Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.

Shi Q, Xie Wl, Zhang B, Chen Ln, Xu Y, Wang K, Ren K, Zhang Xm, Chen C, Zhang J, Dong Xp. Virol J. 2013 Jul 1;10:216. Doi: 10.1186/1743-422X-10-216. Brain Microglia Were Activated In Sporadic Cjd But Almost Unchanged In Fatal Familial Insomnia And...

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6 Customer Q&As for Rat IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-11-20

Question

Q: What is the optimal O.D. value for IL-1 alpha ELISA kit? I performed your IL-1 alpha ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-1 alpha even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-1 alpha ELISA kit to have sensitivity of 0.5pg/ml, that means the minimum amount of IL-1 alpha that can be declared/interpreted as positive by the above standard is 0.5pg/ml.

Boster Scientific Support

Answered: 2019-07-05

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-1 alpha using the EK0390 Rat IL-1 alpha Picokine® ELISA Kit?

D. Rodriguez

Verified customer

Asked: 2019-02-10

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-02-10

Question

Q: Can IL-1 alpha ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

S. Mehta

Verified customer

Asked: 2017-01-08

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-01-08

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2016-04-29

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-04-29

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2014-03-09

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2014-03-09

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