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Product Info Summary
| SKU: | M02285-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD206 Purified MRC1 Monoclonal Antibody
SKU/Catalog Number
M02285-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD206 Purified MRC1 Monoclonal Antibody (Catalog# M02285-1). Tested in Flow Cytometry, IP, WB, IHC-F, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD206 Purified MRC1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02285-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
15-2
Isotype
Mouse IgG1 kappa
Immunogen
Purified human mannose receptor. The mouse monoclonal antibody 15-2 (also known as MR15-2) recognizes an extracellular epitope of CD206 (macrophage mannose receptor, MMR), a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells and hepatic or lymphatic endothelial cells, but not on monocytes.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02285-1 is reactive to MRC1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
166.0 kDa
Background of MRC1
CD206 (macrophage mannose receptor, MMR), also known as mannose receptor C1 (MRC1), is a type I transmembrane glycoprotein serving as pattern recognition receptor for carbogydrate groups on the surface of bacteria, fungi and other pathogens. Expressed mainly on tissue macrophages and dendritic cells, CD206 mediates endocytosis of these pathogens and presentation of their antigens to the adaptive immune system. CD206 can also be detected in a soluble form in human plasma and is elevated in patients with acute sepsis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02285-1 is guaranteed for Flow Cytometry, IP, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Western blotting: 1-2 μg/ml; non-reducing conditions.
Validation Images & Assay Conditions
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Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (concentration in sample 9 µg/ml), GAM PE.
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Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (concentration in sample 9 µg/ml), GAM PE.
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Western blotting analysis of human CD206 using mouse monoclonal antibody 15-2 on lysates of differentiated monocytes (two batches), and on THP-1 and Raji cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD206 was detected around 170 kDa.
Specific Publications For Anti-Hu CD206 Purified MRC1 Monoclonal Antibody (M02285-1)
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