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Product Info Summary
| SKU: | M03470 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-Hu CD305 Purified LAIR1 Monoclonal Antibody
SKU/Catalog Number
M03470
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD305 Purified LAIR1 Monoclonal Antibody (Catalog# M03470). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD305 Purified LAIR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03470)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
NKTA255
Isotype
Mouse IgG1
Immunogen
Activated NK cells and CD3- thymocytes. The mouse monoclonal antibody NKTA255 recognizes an extracellular epitope of CD305 / LAIR1, a 40 kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03470 is reactive to LAIR1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
31.4 kDa
Background of LAIR1
CD305, also known as LAIR1 (leukocyte-associated Ig-like receptor 1), is an inhibitory receptor found on many types of peripheral blood cells. It serves to suppress cell cytotoxicity, activation, proliferation, and differentiation regarding autoantigens via its two intracellular ITIM sites. CD305 belongs to the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family of proteins. It reacts with collagen ligands.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03470 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD305 (NKTA255) purified antibody (concentration in sample 2 µg/ml, GAM APC) and anti-human CD19 (LT19) PE antibody (20 µl reagent / 100 µl of peripheral whole blood).
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Separation of human CD305 positive CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD305 (NKTA255) purified antibody (concentration in sample 2 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD305 (NKTA255) purified antibody (concentration in sample 2 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD305 Purified LAIR1 Monoclonal Antibody (M03470)
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