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Product Info Summary
| SKU: | M00661 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, Functional Assay |
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Product info
Product Name
Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody
SKU/Catalog Number
M00661
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (Catalog# M00661). Tested in Flow Cytometry, IP, IHC-F, Functional Assay application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00661)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, azide-free
Clonality
Monoclonal
Clone Number
1D11
Isotype
Mouse IgG1 kappa
Immunogen
NKL cell line. The mouse monoclonal antibody 1D11 recognizes an extracellular epitope of CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.
Reactive Species
M00661 is reactive to KLRK1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
25.3 kDa
Background of KLRK1
CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00661 is guaranteed for Flow Cytometry, IP, IHC-F, Functional Assay Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Functional application: Blocking of ligand binding.
Immunohistochemistry: 5-10 μg/ml.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 µg/ml) GAM APC and anti-human CD56 (LT56) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).
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Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (M00661)
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