Anti-Hu CD42b Purified GP1BA Monoclonal Antibody

Product Name

Anti-Hu CD42b Purified GP1BA Monoclonal Antibody

View all CD42b/GPIb alpha Antibodies

SKU/Catalog Number

M02073-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (Catalog# M02073-1). Tested in Flow Cytometry, IHC-F application(s). This antibody reacts with Primate, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02073-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

HIP1

Isotype

Mouse IgG1

Immunogen

Peripheral blood mononuclear cells of a patient suffering with CLL. The antibody HIP1 reacts with an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M02073-1 is reactive to GP1BA in Human, Primate

Applications

M02073-1 is guaranteed for Flow Cytometry, IHC-F Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

71.54kDa

Background of CD42b/GPIb alpha

CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of GP1BA using Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (M02073-1).

Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD42b (HIP1) purified antibody (concentration in sample 4 μg/ml) GAM APC.

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Figure 2. Flow Cytometry validation of GP1BA using Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (M02073-1).

Separation of human thrombocytes (red-filled) from CD42b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD42b (HIP1) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Gene/Protein Information For GP1BA (Source: Uniprot.org, NCBI)

Alternative Names

Antigen CD42b-alpha; BP1BA; BSS; CD42b antigen; CD42b; CD42b-alpha; glycoprotein Ib (platelet), alpha polypeptide; Glycoprotein Ibalpha; GP1B; GP1BA; GPIb alpha; GP-Ib alpha; GPIbA; GPIb-alpha; MGC34595; platelet glycoprotein Ib alpha chain; platelet membrane glycoprotein 1b-alpha subunit GP1BA BDPLT1, BDPLT3, BSS, CD42B, CD42b-alpha, DBPLT3, GP1B, GPIbA, GPIbalpha, VWDP glycoprotein Ib platelet subunit alpha platelet glycoprotein Ib alpha chain|GP-Ib alpha|antigen CD42b-alpha|glycoprotein Ib (platelet), alpha polypeptide|glycoprotein Ib platelet alpha subunit|mutant platelet membrane glycoprotein Ib-alpha|platelet membrane glycoprotein 1b-alpha subunit|platelet membrane glycoprotein Ib-alpha

For more info on GP1BA, check out the GP1BA Infographic

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