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Product Info Summary
| SKU: | M09548-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody
SKU/Catalog Number
M09548-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody (Catalog# M09548-1). Tested in Flow Cytometry, WB, IHC-P, IHC-F, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09548-1)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
TB01
Isotype
Mouse IgM
Immunogen
A pool of neuroblastoma cell lines. The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M09548-1 is reactive to B3GAT1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
38.3 kDa
Background of B3GAT1
CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09548-1 is guaranteed for Flow Cytometry, IHC-P, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Immunohistochemistry: 5-10 μg/ml.
Validation Images & Assay Conditions
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Flow cytometry analysis (surface staining) of human buffy coat cells with anti-human CD57 (TB01) purified, GAM-APC.
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Immunohistochemistry staining of human adrenal gland (paraffin-embedded sections) with anti-CD57 (TB01), 10 µg/ml. Commercially tested by LifeSpan BioSciences.
Specific Publications For Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody (M09548-1)
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