Anti-Human BMP5 DyLight® 550 conjugated Antibody

BMP-5 antibody

Boster Bio Anti-Human BMP5 DyLight® 550 conjugated Antibody catalog # A05887-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A05887-Dyl550
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

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Product Name

Anti-Human BMP5 DyLight® 550 conjugated Antibody

View all BMP-5 Antibodies

SKU/Catalog Number

A05887-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human BMP5 DyLight® 550 conjugated Antibody catalog # A05887-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human BMP5 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05887-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BMP5, different from the related mouse sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05887-Dyl550 is reactive to BMP5 in Human

Applications

A05887-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

51.737kDa

Background of BMP-5

Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene. This gene encodes a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. These proteins are synthesized as prepropeptides, cleaved, and then processed into dimeric proteins. And this protein may act as an important signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis. This gene is differentially regulated during the formation of various tumors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For BMP5 (Source: Uniprot.org, NCBI)

Gene Name

BMP5

Full Name

Bone morphogenetic protein 5

Weight

51.737kDa

Superfamily

TGF-beta family

Alternative Names

BMP5; BMP-5; bone morphogenetic protein 5; MGC34244 BMP5 bone morphogenetic protein 5 bone morphogenetic protein 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BMP5, check out the BMP5 Infographic

BMP5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BMP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Human BMP5 DyLight® 550 conjugated Antibody

Question

Is a blocking peptide available for product anti-Human BMP5 DyLight® 550 conjugated antibody (A05887-Dyl550)?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

We do provide the blocking peptide for product anti-Human BMP5 DyLight® 550 conjugated antibody (A05887-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-09

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-Human BMP5 DyLight® 550 conjugated antibody A05887-Dyl550. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-13

Question

We want to test anti-Human BMP5 DyLight® 550 conjugated antibody A05887-Dyl550 on human thymus for research purposes, then I may be interested in using anti-Human BMP5 DyLight® 550 conjugated antibody A05887-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

The products we sell, including anti-Human BMP5 DyLight® 550 conjugated antibody A05887-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-24

Question

Does A05887-Dyl550 anti-Human BMP5 DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

It shows on the product datasheet, A05887-Dyl550 anti-Human BMP5 DyLight® 550 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-05

Question

I have a question about product A05887-Dyl550, anti-Human BMP5 DyLight® 550 conjugated antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05887-Dyl550 anti-Human BMP5 DyLight® 550 conjugated antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-18

Question

Is this A05887-Dyl550 anti-Human BMP5 DyLight® 550 conjugated antibody reactive to the isotypes of BMP5?

Verified Customer

Verified customer

Asked: 2017-09-05

Answer

The immunogen of A05887-Dyl550 anti-Human BMP5 DyLight® 550 conjugated antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human BMP5 (332-365aa HQDSSRMSSVGDYNTSEQKQACKKHELYVSFRDL), different from the related mouse sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-09-05

Question

Please see the WB image, lot number and protocol we used for thymus using anti-Human BMP5 DyLight® 550 conjugated antibody A05887-Dyl550. Please let me know if you require anything else.

J. Li

Verified customer

Asked: 2017-05-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-18

Order DetailsPrice
A05887-Dyl550

100μg

$515
A05887-Dyl550-carrier-free

Carrier Free

$515

Free Secondary Antibody

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A05887-Dyl550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

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