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Product Info Summary
| SKU: | A00978-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CLPX DyLight® 550 conjugated Antibody
SKU/Catalog Number
A00978-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CLPX DyLight® 550 conjugated Antibody catalog # A00978-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CLPX DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00978-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CLPX recombinant protein (Position: A337-E574).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00978-Dyl550 is reactive to CLPX in Human
Applications
A00978-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
69.224kDa
Background of CLPX
ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial is an enzyme that in humans is encoded by the CLPX gene. This protein is a member of the family of AAA Proteins (AAA+ ATPase) and is to form the protein complex of Clp protease. The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CLPX (Source: Uniprot.org, NCBI)
Gene Name
CLPX
Full Name
ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
Weight
69.224kDa
Superfamily
ClpX chaperone family
Alternative Names
ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; ClpX caseinolytic peptidase X homolog (E. coli); ClpX caseinolytic protease X homolog; energy-dependent regulator of proteolysis CLPX EPP2 caseinolytic mitochondrial matrix peptidase chaperone subunit X ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial|ClpX caseinolytic peptidase X homolog|ClpX caseinolytic protease X homolog|energy-dependent regulator of proteolysis
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLPX, check out the CLPX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLPX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CLPX DyLight® 550 conjugated Antibody (A00978-Dyl550)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Human CLPX DyLight® 550 conjugated Antibody
Question
My question regarding product A00978-Dyl550, anti-Human CLPX DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Rodriguez
Verified customer
Asked: 2020-01-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00978-Dyl550 anti-Human CLPX DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-08
Question
I was wanting to use your anti-Human CLPX DyLight® 550 conjugated antibody for Flow Cytometry for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
As indicated on the product datasheet, A00978-Dyl550 anti-Human CLPX DyLight® 550 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-27
Question
Does anti-Human CLPX DyLight® 550 conjugated antibody A00978-Dyl550 work on zebrafish Flow Cytometry with leukemic t-cell?
B. Kulkarni
Verified customer
Asked: 2019-07-12
Answer
Our lab technicians have not validated anti-Human CLPX DyLight® 550 conjugated antibody A00978-Dyl550 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Human CLPX DyLight® 550 conjugated antibody A00978-Dyl550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish leukemic t-cell in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
Would anti-Human CLPX DyLight® 550 conjugated antibody A00978-Dyl550 work for Flow Cytometry with leukemic t-cell?
H. Thomas
Verified customer
Asked: 2019-07-12
Answer
According to the expression profile of leukemic t-cell, CLPX is highly expressed in leukemic t-cell. So, it is likely that anti-Human CLPX DyLight® 550 conjugated antibody A00978-Dyl550 will work for Flow Cytometry with leukemic t-cell.
Boster Scientific Support
Answered: 2019-07-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Human CLPX DyLight® 550 conjugated antibody A00978-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-08
Question
Is a blocking peptide available for product anti-Human CLPX DyLight® 550 conjugated antibody (A00978-Dyl550)?
R. Patel
Verified customer
Asked: 2015-08-31
Answer
We do provide the blocking peptide for product anti-Human CLPX DyLight® 550 conjugated antibody (A00978-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-08-31
Question
Do you have a BSA free version of anti-Human CLPX DyLight® 550 conjugated antibody A00978-Dyl550 available?
A. Huang
Verified customer
Asked: 2013-12-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human CLPX DyLight® 550 conjugated antibody A00978-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-12-10
