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Product Info Summary
| SKU: | A15882 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-DCAMKL3 DCLK3 Antibody
SKU/Catalog Number
A15882
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DCAMKL3 DCLK3 Antibody catalog # A15882. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DCAMKL3 DCLK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15882)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DCLK3. AA range:291-340
Reactive Species
A15882 is reactive to DCLK3 in Human, Mouse
Calculated molecular weight
73.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15882 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using DCAMKL3 Polyclonal Antibody
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Western Blot analysis of Jurkat cells using DCAMKL3 Polyclonal Antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using DCLK3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-DCAMKL3 DCLK3 Antibody (A15882)
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Customer Q&As
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6 Customer Q&As for Anti-DCAMKL3 DCLK3 Antibody
Question
See below the WB image, lot number and protocol we used for tibial nerve using anti-DCAMKL3 antibody A15882. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-20
Question
I have a question about product A15882, anti-DCAMKL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15882 anti-DCAMKL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-03
Question
We are currently using anti-DCAMKL3 antibody A15882 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
The anti-DCAMKL3 antibody (A15882) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-30
Question
I was wanting to use your anti-DCAMKL3 antibody for WB for human tibial nerve on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tibial nerve identification?
Verified Customer
Verified customer
Asked: 2018-08-24
Answer
You can see on the product datasheet, A15882 anti-DCAMKL3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tibial nerve in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-24
Question
Would anti-DCAMKL3 antibody A15882 work on pig WB with tibial nerve?
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
Our lab technicians have not validated anti-DCAMKL3 antibody A15882 on pig. You can run a BLAST between pig and the immunogen sequence of anti-DCAMKL3 antibody A15882 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig tibial nerve in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-31
Question
Does A15882 anti-DCAMKL3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
As indicated on the product datasheet, A15882 anti-DCAMKL3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-31
