Anti-Human PPID DyLight® 550 conjugated Antibody

Cyclophilin 40 antibody

Boster Bio Anti-Human PPID DyLight® 550 conjugated Antibody catalog # A02424-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A02424-Dyl550
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

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Product Name

Anti-Human PPID DyLight® 550 conjugated Antibody

View all Cyclophilin 40 Antibodies

SKU/Catalog Number

A02424-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human PPID DyLight® 550 conjugated Antibody catalog # A02424-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human PPID DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02424-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human PPID recombinant protein (Position: N306-A370).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02424-Dyl550 is reactive to PPID in Human

Applications

A02424-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Background of Cyclophilin 40

Cyclophilin D, Peptidylprolyl isomerase D, also known as PPID, is an enzyme which in humans is encoded by the PPID gene. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. The Cyclophilin D (PPID) gene contains 10 exons and spans 14.2 kb of genomic DNA. By fluorescence in situ hybridization, the PPID gene is mapped to chromosome 4q31.3. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant ciclosporin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For PPID (Source: Uniprot.org, NCBI)

Uniprot ID

Q08752

Gene ID

5481

Gene Name

PPID

Full Name

Peptidyl-prolyl cis-trans isomerase D

Weight

40.764kDa

Superfamily

cyclophilin-type PPIase family

Alternative Names

40 kDa peptidyl-prolyl cis-trans isomerase D; 40 kDa peptidyl-prolyl cis-trans isomerase; cyclophilin D; cyclophilin-40; Cyclophilin-related protein; CYP40; CYP-40cyclophilin 40; CYPD; EC 5.2.1.8; MGC33096; peptidyl-prolyl cis-trans isomerase D; peptidylprolyl isomerase D (cyclophilin D); peptidylprolyl isomerase D; PPIase D; Rotamase D PPID CYP-40, CYPD peptidylprolyl isomerase D peptidyl-prolyl cis-trans isomerase D|40 kDa peptidyl-prolyl cis-trans isomerase D|PPIase D|cyclophilin 40|cyclophilin D|cyclophilin-related protein|rotamase D|testicular tissue protein Li 147

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPID, check out the PPID Infographic

PPID infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPID: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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7 Customer Q&As for Anti-Human PPID DyLight® 550 conjugated Antibody

Question

Is there a BSA free version of anti-Human PPID DyLight® 550 conjugated antibody A02424-Dyl550 available?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Human PPID DyLight® 550 conjugated antibody A02424-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-29

Question

Will A02424-Dyl550 anti-Human PPID DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-05-04

Answer

It shows on the product datasheet, A02424-Dyl550 anti-Human PPID DyLight® 550 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-05-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Human PPID DyLight® 550 conjugated antibody A02424-Dyl550. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-05-03

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-03

Question

Is a blocking peptide available for product anti-Human PPID DyLight® 550 conjugated antibody (A02424-Dyl550)?

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

We do provide the blocking peptide for product anti-Human PPID DyLight® 550 conjugated antibody (A02424-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-09-21

Question

I see that the anti-Human PPID DyLight® 550 conjugated antibody A02424-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

K. Jackson

Verified customer

Asked: 2015-05-08

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-05-08

Question

See below the WB image, lot number and protocol we used for leukemic t-cell using anti-Human PPID DyLight® 550 conjugated antibody A02424-Dyl550. Please let me know if you require anything else.

K. Krishna

Verified customer

Asked: 2014-11-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-11-12

Question

Is this A02424-Dyl550 anti-Human PPID DyLight® 550 conjugated antibody reactive to the isotypes of PPID?

L. Kulkarni

Verified customer

Asked: 2013-06-12

Answer

The immunogen of A02424-Dyl550 anti-Human PPID DyLight® 550 conjugated antibody is E. coli-derived human PPID recombinant protein (Position: N306-A370). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-06-12

Size

Total: $515

SKU:A02424-Dyl550

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A02424-Dyl550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

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