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Product Info Summary
| SKU: | A01614-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human SLK DyLight® 550 conjugated Antibody
SKU/Catalog Number
A01614-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human SLK DyLight® 550 conjugated Antibody catalog # A01614-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human SLK DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01614-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SLK recombinant protein (Position: M1-E232).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01614-Dyl550 is reactive to SLK in Human
Applications
A01614-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
142.695kDa
Background of SLK
STE20-like serine/threonine-protein kinase is an enzyme that in humans is encoded by the SLK gene. This gene is mapped to chromosome 10q24.33-q25.1. SLK is a kinase with multiple functional domains that is predicted to regulate cytoskeletal organization and responses to apoptotic stimuli.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For SLK (Source: Uniprot.org, NCBI)
Gene Name
SLK
Full Name
STE20-like serine/threonine-protein kinase
Weight
142.695kDa
Superfamily
protein kinase superfamily
Alternative Names
STE20-like serine/threonine-protein kinase SLK LOSK, STK2, bA16H23.1, se20-9 STE20 like kinase STE20-like serine/threonine-protein kinase|CTCL tumor antigen se20-9|Long Ste20-like Kinase|SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase|SNF1 sucrose nonfermenting like kinase|STE20-related kinase|STE20-related serine/threonine-protein kinase|Ste20-related serine/threonine kinase|hSLK|serine/threonine-protein kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLK, check out the SLK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human SLK DyLight® 550 conjugated Antibody (A01614-Dyl550)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Human SLK DyLight® 550 conjugated Antibody
Question
I was wanting to use your anti-Human SLK DyLight® 550 conjugated antibody for Flow Cytometry for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
D. Parker
Verified customer
Asked: 2019-11-11
Answer
As indicated on the product datasheet, A01614-Dyl550 anti-Human SLK DyLight® 550 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-11
Question
We are currently using anti-Human SLK DyLight® 550 conjugated antibody A01614-Dyl550 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The anti-Human SLK DyLight® 550 conjugated antibody (A01614-Dyl550) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Human SLK DyLight® 550 conjugated antibody A01614-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-15
Question
I have a question about product A01614-Dyl550, anti-Human SLK DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01614-Dyl550 anti-Human SLK DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-19
Question
Is a blocking peptide available for product anti-Human SLK DyLight® 550 conjugated antibody (A01614-Dyl550)?
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
We do provide the blocking peptide for product anti-Human SLK DyLight® 550 conjugated antibody (A01614-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-04
Question
Does anti-Human SLK DyLight® 550 conjugated antibody A01614-Dyl550 work for Flow Cytometry with liver?
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
According to the expression profile of liver, SLK is highly expressed in liver. So, it is likely that anti-Human SLK DyLight® 550 conjugated antibody A01614-Dyl550 will work for Flow Cytometry with liver.
Boster Scientific Support
Answered: 2017-11-06
Question
I see that the anti-Human SLK DyLight® 550 conjugated antibody A01614-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
C. Gonzalez
Verified customer
Asked: 2016-05-11
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-05-11
