Click image to see more details
Product Info Summary
| SKU: | A00814-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CHM Antibody Picoband®
SKU/Catalog Number
A00814-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CHM Antibody Picoband® catalog # A00814-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CHM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00814-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CHM recombinant protein (Position: A193-Q380).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00814-1 is reactive to CHM in Human, Mouse, Rat
Observed Molecular Weight
73 kDa
Calculated molecular weight
73.5 kDa
Background of CHM
Rab escort protein 1 (REP1) also known as rab proteins geranylgeranyltransferase component A 1 is an enzyme that in humans is encoded by the CHM gene. This gene encodes component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme, this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Rab GTPases need to be geranylgeranyled on either one or two cysteine residues in their C-terminus to localize to the correct intracellular membrane. Mutations in this gene are a cause of choroideremia; also known as tapetochoroidal dystrophy (TCD). This X-linked disease is characterized by progressive dystrophy of the choroid, retinal pigment epithelium and retina. Alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00814-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human A431 whole cell, human 293T whole cell, rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Western blot analysis of CHM using anti-CHM antibody (A00814-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHM antigen affinity purified polyclonal antibody (Catalog # A00814-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHM at approximately 73 kDa. The expected band size for CHM is at 100 kDa.
Specific Publications For Anti-CHM Antibody Picoband® (A00814-1)
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Customer Q&As
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4 Customer Q&As for Anti-CHM Antibody Picoband®
Question
Is a blocking peptide available for product anti-CHM antibody (A00814-1)?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
We do provide the blocking peptide for product anti-CHM antibody (A00814-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-21
Question
Does anti-CHM antibody A00814-1 work on monkey ELISA with retina?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
Our lab technicians have not validated anti-CHM antibody A00814-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CHM antibody A00814-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey retina in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-11
Question
See below the WB image, lot number and protocol we used for retina using anti-CHM antibody A00814-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-16
Question
Is this A00814-1 anti-CHM antibody reactive to the isotypes of CHM?
S. Johnson
Verified customer
Asked: 2017-04-19
Answer
The immunogen of A00814-1 anti-CHM antibody is E. coli-derived human CHM recombinant protein (Position: A193-Q380). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-19
