Anti-IL-17F Antibody Peroxidase Conjugated

Product Name

Anti-IL-17F Antibody Peroxidase Conjugated

View all IL17F Antibodies

SKU/Catalog Number

A02062

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-IL-17F Antibody Peroxidase Conjugated (Catalog # A02062). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-IL-17F Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02062)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

IgG

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.

Reactive Species

A02062 is reactive to IL17F in Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

17.9 kDa

Background of IL17F

IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02062 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000-1:50,000
IHC: 1:500-1:2,500
WB: 1:1,000-1:5,000
This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 17.9 kDa in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Validation Images & Assay Conditions

See full target information IL17F

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