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Product Info Summary
| SKU: | A00857-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-IL-27 Antibody
SKU/Catalog Number
A00857-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IL-27 Antibody (Catalog # A00857-3). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse.
Storage & Handling
IL-27 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IL-27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00857-3)
Host
Rabbit
Contents
IL-27 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IL-27 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human IL-27. The immunogen is located within the first 50 amino acids of IL-27.
Reactive Species
A00857-3 is reactive to IL27 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.5 kDa
Background of IL27
Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00857-3 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5 - 4 μg/mL; ICC: 10 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Mouse A20 Cells
Loading: 15 μg of lysates per lane.
Antibodies: IL-27 A00857-3, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: IL-27 A00857-3 (4 μg/mL), IL-27 3799 (4 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Recombinant Protein Validation
Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane.
Antibodies: IL-27 A00857-3, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane 1: 0.5 μg/mL,
Lane 2: 1 μg/mL,
Lane 3: 2 μg/mL
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Immunocytochemistry Validation of IL-27 in Mouse EL4 Cells
Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody (A00857-3) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-IL-27 Antibody (A00857-3)
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