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- Table of Contents
IL27 antibody (A00857-3) performs well in ELISA, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunocytochemistry Validation of IL-27 in Mouse EL4 Cells
Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody (A00857-3) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody (A00857-3) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Designing a Western blot for IL27? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the IL27 Western Blot GuideBundle Save
Anti-IL-27 Antibody
IL27 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 0.5 - 4 μg/mL; ICC: 10 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IL-27 Antibody
View all IL27 Antibodies
View all IL27 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
IL-27 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
IL-27 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IL-27 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human IL-27. The immunogen is located within the first 50 amino acids of IL-27.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
27.5 kDa
Background of IL27
Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.
Cite This Product
Anti-IL-27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00857-3)
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Product Promise
We promise Anti-IL-27 Antibody performs excellently in relevant applications for ELISA, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00857-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 28, 2026