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Product Info Summary
| SKU: | M00101 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL1 beta Rabbit Monoclonal Antibody
SKU/Catalog Number
M00101
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IL1 beta Rabbit Monoclonal Antibody catalog # M00101. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL1 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00101)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IBF-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IL1 beta
Reactive Species
M00101 is reactive to IL1B in Human
Observed Molecular Weight
17 kDa
Calculated molecular weight
30.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00101 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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Western blot analysis of IL1 beta expression in recombinant IL1 beta protein.
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Western blot analysis of IL1 beta using anti-IL1 beta antibody ( M00101).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-6: mouse PACs whole cell lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (M00101) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system.The expected band size for IL1 beta is at 30 kDa.
Specific Publications For Anti-IL1 beta Rabbit Monoclonal Antibody (M00101)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-IL1 beta Rabbit Monoclonal Antibody
Question
See below the WB image, lot number and protocol we used for skin using anti-IL1 beta Rabbit Monoclonal antibody M00101. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-23
Question
Is there a BSA free version of anti-IL1 beta Rabbit Monoclonal antibody M00101 available?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL1 beta Rabbit Monoclonal antibody M00101 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-04
Question
I was wanting to use your anti-IL1 beta Rabbit Monoclonal antibody for WB for human skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skin identification?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
As indicated on the product datasheet, M00101 anti-IL1 beta Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-09
Question
I see that the anti-IL1 beta Rabbit Monoclonal antibody M00101 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-12
Question
My question regarding product M00101, anti-IL1 beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00101 anti-IL1 beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-09
Question
My lab would like to test anti-IL1 beta Rabbit Monoclonal antibody M00101 on human skin for research purposes, then I may be interested in using anti-IL1 beta Rabbit Monoclonal antibody M00101 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The products we sell, including anti-IL1 beta Rabbit Monoclonal antibody M00101, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-28
Question
Does anti-IL1 beta Rabbit Monoclonal antibody M00101 work for WB with skin?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
According to the expression profile of skin, IL1B is highly expressed in skin. So, it is likely that anti-IL1 beta Rabbit Monoclonal antibody M00101 will work for WB with skin.
Boster Scientific Support
Answered: 2019-08-14
Question
Is a blocking peptide available for product anti-IL1 beta Rabbit Monoclonal antibody (M00101)?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
We do provide the blocking peptide for product anti-IL1 beta Rabbit Monoclonal antibody (M00101). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-04
Question
My colleagues were happy with the WB result of your anti-IL1 beta Rabbit Monoclonal antibody. However we have been able to see positive staining in monocyte cytosol using this antibody. Is that expected? Could you tell me where is IL1B supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
According to literature, monocyte does express IL1B. Generally IL1B expresses in cytoplasm, cytosol. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2019-06-13
Question
Is this M00101 anti-IL1 beta Rabbit Monoclonal antibody reactive to the isotypes of IL1B?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
The immunogen of M00101 anti-IL1 beta Rabbit Monoclonal antibody is A synthesized peptide derived from human IL1 beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-10
Question
We are currently using anti-IL1 beta Rabbit Monoclonal antibody M00101 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
The anti-IL1 beta Rabbit Monoclonal antibody (M00101) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-16
Question
Will M00101 anti-IL1 beta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-10
Answer
As indicated on the product datasheet, M00101 anti-IL1 beta Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-10
Question
We have been able to see staining in human macrophage. Any tips? Is anti-IL1 beta Rabbit Monoclonal antibody supposed to stain macrophage positively?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
Based on literature macrophage does express IL1B. Based on Uniprot.org, IL1B is expressed in smooth muscle tissue, leukocyte, histiocytic lymphoma, monocyte, lung, skin, macrophage, among other tissues. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2017-07-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-IL1 beta Rabbit Monoclonal antibody M00101. Let me know if you need anything else.
R. Wu
Verified customer
Asked: 2014-08-12
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-12
