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- Table of Contents
ING5 antibody (A04974-3) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Monkey.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of JK cells using anti-ING5 antibody (A04974-3).
Overlay histogram showing JK cells stained with A04974-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ING5 Antibody (A04974-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing JK cells stained with A04974-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ING5 Antibody (A04974-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-ING5 Antibody Picoband®
ING5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
ING5 antibody was positively detected by WB in:
human Jurkat whole cell, human PC-3 whole cell, human 293T whole cell, monkey COS-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ING5 antibody was positively detected by ICC/IF in:
SiHa cell
ING5 antibody was positively detected by FC in:
JK cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human, Monkey
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Tested
Recommend
Dog
Predicted
Predicted
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-ING5 Antibody Picoband®
View all ING5 Antibodies
View all ING5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human ING5 recombinant protein (Position: K38-K82). Human ING5 shares 91.1% amino acid (aa) sequence?identity with mouse ING5.
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
32 kDa
Calculated Molecular Weight
27.8 kDa
Background of ING5
Inhibitor of growth protein 5 is a protein that in humans is encoded by the ING5 gene. This gene encodes a tumor suppressor protein that inhibits cell growth and induces apoptosis. This protein contains a PHD-type zinc finger. It interacts with tumor suppressor p53 and p300, a component of the histone acetyl transferase complex, suggesting a role in transcriptional regulation. Alternative splicing and the use of multiple promoters and 3' terminal exons results in multiple transcript variants.
Cite This Product
Anti-ING5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04974-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
96%
High Homology
Mouse
91%
High Homology
Rat
91%
High Homology
Rabbit
91%
High Homology
Cow
91%
High Homology
Sheep
91%
High Homology
Zebrafish
76%
Moderate Homology
Drosophila
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ING5 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ING5 antibody
Flow Cytometry (FC) protocol for ING5 antibody
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Product Promise
We promise Anti-ING5 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A04974-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026