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Product Info Summary
| SKU: | A03354 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IPR1 SP110 Antibody
SKU/Catalog Number
A03354
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IPR1 SP110 Antibody (Catalog # A03354). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IPR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IPR1 SP110 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03354)
Host
Rabbit
Contents
IPR1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IPR1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human IPR1. The immunogen is located within amino acids 470 - 520 of IPR1.
Reactive Species
A03354 is reactive to SP110 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
78.4 kDa
Background of SP110
Susceptibility to tuberculosis (TB) in mice has recently been attributed to the IPR1 gene. IPR1 is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified. SP110 is the closest homolog of the IPR1 protein in humans. The IPR1/Sp110 gene product might play a role in integrating signals generated by intracellular pathogens with mechanisms controlling innate immunity, cell death, and pathogenesis. IPR1/Sp110 is up-regulated after infection with M. tuberculosis and required by Anaplasma phagocytophilum for infection of human promyelocytic cells. Defects in Sp110 are a cause of severely impaired resistance to infection by M. tuberculosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03354 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Ipr1 antibody can be used for detection of Ipr1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IPR1 in Hela cell lysate with IPR1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
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Immunohistochemical staining of human brain tissue using IPR1 antibody at 2.5 μg/mL.
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Immunofluorescence of IPR1 in Human Brain cells with IPR1 antibody at 50 μg/mL.
Specific Publications For Anti-IPR1 SP110 Antibody (A03354)
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